Table of Contents - *138330
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Genome
DNA
Protein
Gene Info
Variation
Animal Models
Cellular Pathways
| *138330 | ||||||||||||
| GLUTATHIONE S-TRANSFERASE, MICROSOMAL, 1; MGST1 | ||||||||||||
| Alternative titles; symbols | ||||||||||||
| GLUTATHIONE S-TRANSFERASE 12; GST12 | ||||||||||||
| HGNC Approved Gene Symbol: MGST1 | ||||||||||||
| Cytogenetic location: 12p12.3 Genomic coordinates (GRCh37): 12:16,500,075 - 16,517,343 (from NCBI) | ||||||||||||
| TEXT | ||||||||||||
| The glutathione S-transferases are products of a gene superfamily. These dimeric proteins catalyze the conjugation of glutathione with a variety of xenobiotics and their reactive metabolites. They are composed of 3 classes of subunits according to protein sequence similarity and antibody crossreactivity: alpha, mu, and pi. A fourth GST is a unique membrane-bound transferase that is structurally distinct from the cytosolic forms. It has no amino acid or nucleic acid sequence homology to the cytosolic enzymes and may function as a trimer rather than as a dimer. By screening a human liver cDNA library with a rat microsomal GST cDNA, DeJong et al. (1988) identified cDNAs encoding MGST1. Northern blot analysis revealed that MGST1 is expressed as a 0.95-kb mRNA in liver. The predicted 154-amino acid protein shares 83% amino acid identity with rat microsomal GST. Using the MGST1 cDNA, DeJong et al. (1989, 1990) characterized the MGST1 gene by genomic blotting and assigned the gene to chromosome 12 by study of a panel of mouse-human somatic cell hybrid DNAs. Kelner et al. (1996) determined that the GST12 gene spans 12.8 kb and comprises 4 exons and 3 introns. The entire coding sequence resides on exons 2, 3, and 4. Kelner et al. (1996) also confirmed the presence of an upstream false start and a stop codon which could produce a heptadecapeptide and depress translation initiation at the authentic AUG. The authors stated, however, that initiation is suppressed at the false start codon due to either the closeness of the upstream stop codon or the suboptimal context of the codon. | ||||||||||||
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