Table of Contents - *607737
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Genome
DNA
Protein
Gene Info
Variation
Animal Models
Cellular Pathways
| *607737 | ||||||
| FIBROBLAST GROWTH FACTOR-BINDING PROTEIN 1; FGFBP1 | ||||||
| Alternative titles; symbols | ||||||
| FGF-BINDING PROTEIN 1; FGFBP HEPARIN-BINDING GROWTH FACTOR-BINDING PROTEIN, 17-KD; HBP17 HBGF-BINDING PROTEIN, 17-KD | ||||||
| HGNC Approved Gene Symbol: FGFBP1 | ||||||
| Cytogenetic location: 4p15.32 Genomic coordinates (GRCh37): 4:15,937,191 - 15,939,970 (from NCBI) | ||||||
| TEXT | ||||||
| Description | ||||||
| FGFBP1, or HBP17, binds to both acidic (FGF1; 131220) and basic (FGF2; 134920) fibroblast growth factors in a reversible manner. It also binds to perlecan (HSPG2; 142461). | ||||||
| Cloning | ||||||
| Using affinity chromatography and biochemical analysis, followed by PCR of an epidermoid carcinoma cell line, Wu et al. (1991) isolated a cDNA encoding HBP17. The predicted 234-amino acid secreted protein contains a signal sequence and a potential N-linked glycosylation site. Northern blot analysis revealed expression of a 1.2-kb transcript in epidermoid and squamous carcinoma cells, as well as in normal keratinocytes. Expression was not detected in any other cell lines tested, including nontumorigenic epidermoid cell lines. Immunoblot analysis detected a 17-kD protein that comigrated with FGF2. | ||||||
| Gene Function | ||||||
| By binding analyses, Wu et al. (1991) showed that HBP17 interacts with both FGF1 and FGF2. Like heparin, HBP17 protected both FGF molecules from acid inactivation. HBP17 inhibited the mitogenic activity of FGF1 more efficiently than that of FGF2 in the absence of heparin, and it blocked binding to a liver cell line. By yeast 2-hybrid and coimmunoprecipitation analyses with domain III of HSPG2 as bait, Mongiat et al. (2001) showed that keratinocyte FGFBP1 interacts with HSPG2. Deletion analysis determined that FGFBP1 binds to the second EGF motif of domain III, close to the binding site for FGF7 (148180). Immunohistochemical analysis demonstrated low-level expression of FGFBP1 in the suprabasal region of the epidermis and higher expression and colocalization with HSPG2 in the pericellular stroma of squamous cell carcinomas. | ||||||
| Mapping | ||||||
| The International Radiation Hybrid Mapping Consortium mapped the FGFBP1 gene to chromosome 4 (D4S3140). | ||||||
| REFERENCES | ||||||
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