Entry - *194538 - ZINC FINGER PROTEIN 10; ZNF10 - OMIM

 
 
* 194538

ZINC FINGER PROTEIN 10; ZNF10


Alternative titles; symbols

KOX1


HGNC Approved Gene Symbol: ZNF10

Cytogenetic location: 12q24.33   Genomic coordinates (GRCh38) : 12:133,130,627-133,159,465 (from NCBI)


TEXT

▼ Cloning and Expression

Using the zinc finger region of mouse Mkr1 (ZFP1; 617230) to screen a human T-cell line cDNA library, Thiesen (1990) cloned ZNF10, which he called KOX1. The deduced 462-amino acid protein has a calculated molecular mass of at least 50 kD. It has 11 consecutive zinc fingers containing conserved cys/his residues, with variations in fingers 1 and 11. Northern blot analysis showed variable KOX1 expression in several human cell lines, with highest expression in the U937 myelomonocytic cell line. Several KOX1 transcripts were detected, suggesting it may be alternatively spliced.


▼ Gene Function

Using a zinc blot, Thiesen (1990) demonstrated that KOX1 specifically bound zinc.


▼ Mapping

In the course of mapping 27 nonoverlapping zinc finger cDNAs from human T cells by analysis of somatic cell hybrids, Huebner et al. (1991) mapped ZNF10 to chromosome 12q13-qter, probably clustered with ZNF26 (194537). Rousseau-Merck et al. (1993) also mapped the ZNF10 gene to chromosome 12q24.33 and demonstrated that it and ZNF26 are located within a pulsed field gel electrophoresis fragment less than 300 kb long. The mapping was done by a combination of somatic cell hybridization and in situ hybridization. Since ZNF26 has been mapped to 12q24.33 by in situ hybridization, this also must be the localization of ZNF10.


▼ REFERENCES

  1. Huebner, K., Druck, T., Croce, C. M., Thiesen, H. J. Twenty-seven nonoverlapping zinc finger cDNAs from human T cells map to nine different chromosomes with apparent clustering. Am. J. Hum. Genet. 48: 726-740, 1991. [PubMed: 2014798, related citations]

  2. Rousseau-Merck, M.-F., Hillion, J., Jonveaux, P., Couillin, P., Seite, P., Thiesen, H.-J., Berger, R. Chromosomal localization of 9 KOX zinc finger genes: physical linkages suggest clustering of KOX genes on chromosomes 12, 16, and 19. Hum. Genet. 92: 583-587, 1993. [PubMed: 8262519, related citations] [Full Text]

  3. Thiesen, H.-J. Multiple genes encoding zinc finger domains are expressed in human T cells. New Biologist 2: 363-374, 1990. [PubMed: 2288909, related citations]


Contributors:
Patricia A. Hartz - updated : 8/8/2007
Creation Date:
Victor A. McKusick : 7/7/1992
Edit History:
mgross : 11/30/2016
mgross : 08/13/2007
terry : 8/8/2007
dkim : 6/25/1998
mark : 5/14/1996
carol : 2/24/1994
carol : 7/7/1992

* 194538

ZINC FINGER PROTEIN 10; ZNF10


Alternative titles; symbols

KOX1


HGNC Approved Gene Symbol: ZNF10

Cytogenetic location: 12q24.33   Genomic coordinates (GRCh38) : 12:133,130,627-133,159,465 (from NCBI)


TEXT

Cloning and Expression

Using the zinc finger region of mouse Mkr1 (ZFP1; 617230) to screen a human T-cell line cDNA library, Thiesen (1990) cloned ZNF10, which he called KOX1. The deduced 462-amino acid protein has a calculated molecular mass of at least 50 kD. It has 11 consecutive zinc fingers containing conserved cys/his residues, with variations in fingers 1 and 11. Northern blot analysis showed variable KOX1 expression in several human cell lines, with highest expression in the U937 myelomonocytic cell line. Several KOX1 transcripts were detected, suggesting it may be alternatively spliced.


Gene Function

Using a zinc blot, Thiesen (1990) demonstrated that KOX1 specifically bound zinc.


Mapping

In the course of mapping 27 nonoverlapping zinc finger cDNAs from human T cells by analysis of somatic cell hybrids, Huebner et al. (1991) mapped ZNF10 to chromosome 12q13-qter, probably clustered with ZNF26 (194537). Rousseau-Merck et al. (1993) also mapped the ZNF10 gene to chromosome 12q24.33 and demonstrated that it and ZNF26 are located within a pulsed field gel electrophoresis fragment less than 300 kb long. The mapping was done by a combination of somatic cell hybridization and in situ hybridization. Since ZNF26 has been mapped to 12q24.33 by in situ hybridization, this also must be the localization of ZNF10.


REFERENCES

  1. Huebner, K., Druck, T., Croce, C. M., Thiesen, H. J. Twenty-seven nonoverlapping zinc finger cDNAs from human T cells map to nine different chromosomes with apparent clustering. Am. J. Hum. Genet. 48: 726-740, 1991. [PubMed: 2014798]

  2. Rousseau-Merck, M.-F., Hillion, J., Jonveaux, P., Couillin, P., Seite, P., Thiesen, H.-J., Berger, R. Chromosomal localization of 9 KOX zinc finger genes: physical linkages suggest clustering of KOX genes on chromosomes 12, 16, and 19. Hum. Genet. 92: 583-587, 1993. [PubMed: 8262519] [Full Text: https://doi.org/10.1007/BF00420943]

  3. Thiesen, H.-J. Multiple genes encoding zinc finger domains are expressed in human T cells. New Biologist 2: 363-374, 1990. [PubMed: 2288909]


Contributors:
Patricia A. Hartz - updated : 8/8/2007

Creation Date:
Victor A. McKusick : 7/7/1992

Edit History:
mgross : 11/30/2016
mgross : 08/13/2007
terry : 8/8/2007
dkim : 6/25/1998
mark : 5/14/1996
carol : 2/24/1994
carol : 7/7/1992



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: Nov. 17, 2024