HGNC Approved Gene Symbol: ZNF70
Cytogenetic location: 22q11.23 Genomic coordinates (GRCh38) : 22:23,738,682-23,751,112 (from NCBI)
Watanabe et al. (2016) stated that ZNF70 is a Kruppel C2H2-type zinc finger protein that localizes to the nucleus and cytoplasm.
Using coimmunoprecipitation in HEK293T cells, Watanabe et al. (2016) demonstrated that ZNF70 interacts with ZFP64 (618111), a transactivator of HES1 (139605). Overexpression of ZNF70 caused a 1.2- to 1.5-fold increase in HES1 expression in HepG2 cells. Cotransfection of ZNF70 and ZFP64 caused a 2-fold increase in HES1 expression. ChIP assays in HEK293T cells showed that both ZFP64 and ZNF70 bind to the HES1 promoter, and cotransfection of ZFP64 and ZNF70 additively stimulated the HES1 promoter. Using tandem affinity purification and coimmunoprecipitation in HEK293T cells, Watanabe et al. (2016) demonstrated that ILDR2 (618081) interacts with ZNF70 but not with ZFP64. Immunoprecipitation showed that this interaction requires exons 6 through 8 of the ILDR2 C terminus. ILDR2 knockdown in HepG2 cells resulted in translocation of both ZNF70 and ZFP64 to the nucleus and a subsequent increase in HES1 expression.
By hybridization to a panel of somatic cell hybrids containing various deletions of chromosome 22, Aubry et al. (1992) mapped the genes encoding 2 zinc finger proteins to the short arm of chromosome 22 and 4 others, including ZNF70, to the 22q11.2 region. Hitherto, the short arms of acrocentric chromosomes were thought to encode only ribosomal RNA genes.
Aubry, M., Marineau, C., Zhang, F. R., Zahed, L., Figlewicz, D., Delattre, O., Thomas, G., de Jong, P. J., Julien, J.-P., Rouleau, G. A. Cloning of six new genes with zinc finger motifs mapping to short and long arms of human acrocentric chromosome 22 (p and q11.2). Genomics 13: 641-648, 1992. [PubMed: 1639391] [Full Text: https://doi.org/10.1016/0888-7543(92)90135-f]
Watanabe, K., Nakayama, K., Ohta, S., Tago, K., Boonvisut, S., Millings, E. J., Fischer, S. G., LeDuc, C. A., Leibel, R. L., Iwamoto, S. ZNF70, a novel ILDR2-interacting protein, contributes to the regulation of HES1 gene expression. Biochem. Biophys. Res. Comm. 477: 712-716, 2016. [PubMed: 27353377] [Full Text: https://doi.org/10.1016/j.bbrc.2016.06.124]