HGNC Approved Gene Symbol: HMGN5
Cytogenetic location: Xq21.1 Genomic coordinates (GRCh38) : X:81,113,699-81,201,913 (from NCBI)
Shirakawa et al. (2000) cloned a mouse cDNA, designated Nsbp1, encoding a protein with similarities to the high mobility group proteins HMG14 (163920) and HMG17 (163910). Using a cDNA clone isolated from a bone marrow stromal cell (BMSC) cDNA library (Jia et al., 1998), King and Francomano (2001) cloned the human homolog from an infant brain cDNA library. The full-length NSBP1 cDNA encodes a 282-amino acid polypeptide that shares 86% sequence similarity with the mouse protein. Like HMG14 and HMG17, the mouse and human proteins have positively charged N-terminal domains and negatively charged C-terminal domains as well as 2 highly conserved motifs in the N terminus: a nuclear localization signal and an HMG14/HMG17 signature in a nucleosomal binding domain. The human and mouse proteins differ from each other in the length and composition of the C terminus, which is associated with transcriptional activation in the mouse. Northern blot analysis of human tissues detected expression in adult heart, brain, liver, kidney, pancreas, trabecular bone fibroblasts, and BMSCs and in fetal liver and kidney. NSBP1 transcripts were detected in fetal heart and brain by RT-PCR but not by Northern blot analysis. The level of expression of NSBP1 transcripts of 1.9, 1.5, and 1.7 kb, resulting from alternative polyadenylation, appeared to vary among tissues.
King and Francomano (2001) determined that the single-copy NSBP1 gene contains 6 exons and spans approximately 8.6 kb of genomic DNA.
By radiation hybrid analysis and FISH, King and Francomano (2001) mapped the NSBP2 gene to chromosome Xq13, proximal to DXS983 and distal to DXS995. By radiation hybrid analysis, they mapped the mouse homolog to the X chromosome.
Jia, L., Wilkin, D., King, L. M., Young, M., Rossi deRubeis, A., Powell, J., Yang, L., Robey, P., Francomano, C. A. Bone marrow cells and trabecular bone cells: comparative functional genomics. (Abstract) Am. J. Hum. Genet. 63: A250, 1998.
King, L. M., Francomano, C. A. Characterization of a human gene encoding nucleosomal binding protein NSBP1. Genomics 71: 163-173, 2001. [PubMed: 11161810] [Full Text: https://doi.org/10.1006/geno.2000.6443]
Shirakawa, H., Landsman, D., Postnikov, Y. V., Bustin, M. NBP-45, a novel nucleosomal binding protein with a tissue-specific and developmentally regulated expression. J. Biol. Chem. 275: 6368-6374, 2000. [PubMed: 10692437] [Full Text: https://doi.org/10.1074/jbc.275.9.6368]