Alternative titles; symbols
HGNC Approved Gene Symbol: KYAT1
Cytogenetic location: 9q34.11 Genomic coordinates (GRCh38) : 9:128,832,942-128,882,523 (from NCBI)
Cysteine conjugate beta-lyase (glutamine transaminase K; kynurenine aminotransferase; EC 2.6.1.64) metabolizes cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites that can produce nephrotoxicity and neurotoxicity in experimental animals and man (Perry et al., 1995).
Using a combination of hybridization screening and PCR techniques, Perry et al. (1995) isolated a full-length cDNA for human kidney cysteine conjugate beta-lyase, or CCBL1. Comparison of the deduced amino acid sequence with that of the rat enzyme indicated an 82% overall similarity, with 90% similarity around the pyridoxal phosphate-binding site. Many of the changes were conservative in nature.
Perry et al. (1995) found that expression of CCBL1 cDNA in COS-1 cells resulted in production of a cytosolic enzyme that showed both cysteine conjugate beta-lyase and glutamine transaminase K activity.
Yu et al. (2006) noted that Kat2 -/- mice have lower hippocampal kynurenic acid levels and are hyperactive compared with wildtype mice, but these abnormalities occur early and are transitory. Yu et al. (2006) found that Kat1 and Kat3 (CCBL2; 610656) expression, when measured together, was higher in Kat2 -/- mice at postnatal day 60, but not at postnatal days 7, 14, 21, or 28, compared with wildtype mice. The increased Kat1 and Kat3 expression paralleled restoration of the normal phenotype in Kat2 -/- mice. Yu et al. (2006) concluded that upregulation of Kat1 and Kat3 compensates for loss of Kat2 in Kat2 -/- mice.
By PCR analysis of genomic DNA from human/rodent hybrid cells, Perry et al. (1995) mapped the CCBL1 gene to chromosome 9. By database analysis, Yu et al. (2006) mapped the CCBL1 gene to chromosome 9q34.11.
Perry, S., Harries, H., Scholfield, C., Lock, T., King, L., Gibson, G., Goldfarb, P. Molecular cloning and expression of a cDNA for human kidney cysteine conjugate beta-lyase. FEBS Lett. 360: 277-280, 1995. [PubMed: 7883047] [Full Text: https://doi.org/10.1016/0014-5793(95)00123-q]
Yu, P., Li, Z., Zhang, L., Tagle, D. A., Cai, T. Characterization of kynurenine aminotransferase III, a novel member of a phylogenetically conserved KAT family. Gene 365: 111-118, 2006. [PubMed: 16376499] [Full Text: https://doi.org/10.1016/j.gene.2005.09.034]