HGNC Approved Gene Symbol: ESM1
Cytogenetic location: 5q11.2 Genomic coordinates (GRCh38) : 5:54,977,867-54,985,593 (from NCBI)
Lassalle et al. (1996) identified a human endothelial cell-specific molecule, ESM1, in a human umbilical vein endothelial (HUVEC) cell cDNA library. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue was restricted to lung. Immunoblot and immunoprecipitation analysis identified a 20-kD protein.
Leukocyte migration involves signal molecules that are present on or produced by endothelial cells, and display of these signals is influenced by cytokines. Lassalle et al. (1996) investigated the effect of several cytokines on ESM1. A time-dependent upregulation of ESM1 mRNA was seen after addition of tumor necrosis factor-alpha (TNFA; 191160) or interleukin-1-beta (IL1B; 147720), but not with interleukin-4 (IL4; 147780) or interferon-gamma (IFNG; 147570) alone. When IFNG was combined with TNFA, IFNG inhibited the TNFA-induced increase of ESM1 mRNA level.
Gross (2014) mapped the ESM1 gene to chromosome 5q11.2 based on an alignment of the ESM1 sequence (GenBank BC011989) with the genomic sequence (GRCh37).
Gross, M. B. Personal Communication. Baltimore, Md. 5/14/2014.
Lassalle, P., Molet, S., Janin, A., Van der Heyden, J., Tavernier, J., Fiers, W., Devos, R., Tonnel, A.-B. ESM-1 is a novel human endothelial cell-specific molecule expressed in lung and regulated by cytokines. J. Biol. Chem. 271: 20458-20464, 1996. [PubMed: 8702785] [Full Text: https://doi.org/10.1074/jbc.271.34.20458]