Alternative titles; symbols
HGNC Approved Gene Symbol: DPF3
Cytogenetic location: 14q24.2 Genomic coordinates (GRCh38) : 14:72,609,034-72,894,101 (from NCBI)
Chestkov et al. (1996) described DPF3, which they called Cer-d4, a conserved gene homologous to Neuro-d4 (DPF1; 601670). The human protein is the apparent homolog of the mouse Cer-d4 protein.
Ninkina et al. (2001) isolated a cDNA encoding a truncated isoform of mouse Dpf3, which they called Cerd4. The deduced protein contains 356 amino acids. Northern blot analysis detected multiple Cerd4 transcripts in mouse retina, cerebellum, and testis only.
Lange et al. (2008) stated that there are 2 splice variants of DPF3. They cloned the cDNA encoding the full-length isoform, DPF3B, from a human heart cDNA library. The deduced 378-amino acid DPF3B protein contains an N-terminal 2/3 domain, which includes a putative nuclear localization signal, followed by a nuclear receptor interaction domain, a C2H2 Kruppel-like zinc finger, and 2 C-terminal plant homeodomains (PHDs). The deduced truncated isoform, DPF3A, contains 357 amino acids and is identical to DPF3B through the first 290 amino acids, but it contains only a single truncated PHD finger at its C terminus.
Zeng et al. (2010) reported the structural mechanism of acetylated histone binding by the double PHD fingers of human DPF3B. Their 3-dimensional solution structure and biochemical analysis of DPF3B highlighted the molecular basis of the integrated tandem PHD finger, which acts as 1 functional unit in the sequence-specific recognition of lys14-acetylated histone H3 (see 602810). Whereas the interaction with H3 was promoted by acetylation at lys14, it was inhibited by methylation at lys4, and these opposing influences were important during transcriptional activation of the mouse Dpb3b target genes Pitx2 (601542) and Jmjd1c (604503). Zeng et al. (2010) concluded that binding of this tandem protein module to chromatin can thus be regulated by different histone modifications during the initiation of gene transcription.
Ninkina et al. (2001) determined that the DPF3 gene contains 12 exons and spans over 275 kb.
Chestkov et al. (1996) mapped the DPF3 gene to chromosome 14q24.3-q31.1 by fluorescence in situ hybridization.
Ninkina et al. (2001) mapped the mouse Dpf3 gene to a region of chromosome 12D3 that shares homology of synteny with human chromosome 14q24.3-q31.
Chestkov, A. V., Baka, I. D., Kost, M. V., Georgiev, G. P., Buchman, V. L. The d4 gene family in the human genome. Genomics 36: 174-177, 1996. [PubMed: 8812431] [Full Text: https://doi.org/10.1006/geno.1996.0440]
Lange, M., Kaynak, B., Forster, U. B., Tonjes, M., Fischer, J. J., Grimm, C., Schlesinger, J., Just, S., Dunkel, I., Krueger, T., Mebus, S., Lehrach, H., Lurz, R., Gobom, J., Rottbauer, W., Abdelilah-Seyfried, S., Sperling, S. Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex. Genes Dev. 22: 2370-2384, 2008. [PubMed: 18765789] [Full Text: https://doi.org/10.1101/gad.471408]
Ninkina, N. N., Mertsalov, I. B., Kulikova, D. A., Alimova-Kost, M. V., Simonova, O. B., Korochkin, L. I., Kiselev, S. L., Buchman, V. L. Cerd4, third member of the d4 gene family: expression and organization of genomic locus. Mammalian Genome 12: 862-866, 2001. [PubMed: 11845289] [Full Text: https://doi.org/10.1007/s00335-001-3039-1]
Zeng, L., Zhang, Q., Li, S., Plotnikov, A. N., Walsh, M. J., Zhou, M.-M. Mechanism and regulation of acetylated histone binding by the tandem PHD finger of DPF3b. Nature 466: 258-262, 2010. [PubMed: 20613843] [Full Text: https://doi.org/10.1038/nature09139]