HGNC Approved Gene Symbol: CLK3
Cytogenetic location: 15q24.1 Genomic coordinates (GRCh38) : 15:74,608,372-74,630,201 (from NCBI)
Serine/threonine protein kinases are a family of enzymes that catalyze the phosphorylation of proteins at serine and threonine residues. Hanes et al. (1994) cloned human ovarian follicle cDNAs encoding the novel serine/threonine kinase CLK3. They identified 2 alternative CLK3 cDNAs of different lengths that correspond to transcripts coexisting in human prostate, testis, leukocytes, and muscle. The longer CLK3 cDNA encodes a predicted 490-amino acid protein that has a nonconserved N-terminal domain, a highly conserved C-terminal kinase domain, and multiple potential phosphorylation sites. This CLK3 isoform has 61% and 51% sequence identity with the CLK2 (602989) and CLK1 (CLK; 601951) proteins, respectively. The shorter cDNA contains an internal deletion of 97 bp, resulting in a predicted 152-amino acid protein that lacks the kinase domain. Southern blot analysis of human genomic DNA suggested that CLK3 is a single-copy gene.
By PCR analysis of a human-rodent somatic cell hybrid panel, Hanes et al. (1994) mapped the human CLK3 gene to chromosome 15. Talmadge et al. (1998) mapped the CLK3 gene to chromosome 15q24 by fluorescence in situ hybridization.
Hanes, J., von der Kammer, H., Klaudiny, J., Scheit, K. H. Characterization by cDNA cloning of two new human protein kinases: evidence by sequence comparison of a new family of mammalian protein kinases. J. Molec. Biol. 244: 665-672, 1994. [PubMed: 7990150] [Full Text: https://doi.org/10.1006/jmbi.1994.1763]
Talmadge, C. B., Finkernagel, S., Sumegi, J., Sciorra, L., Rabinow, L. Chromosomal mapping of three human LAMMER protein-kinase-encoding genes. Hum. Genet. 103: 523-524, 1998. [PubMed: 9856501] [Full Text: https://doi.org/10.1007/s004390050861]