HGNC Approved Gene Symbol: ZNF189
Cytogenetic location: 9q31.1 Genomic coordinates (GRCh38) : 9:101,398,851-101,410,654 (from NCBI)
Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type. Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present (summary by Odeberg et al., 1998).
Odeberg et al. (1998) cloned human bone marrow cDNAs representing 4 differentially spliced ZNF189 transcripts that differ in their 5-prime coding regions. The exons encode a predicted KRAB A domain at the N terminus, a spacer region, and 16 zinc finger motifs at the C terminus; all identified splice variants encode the spacer region and zinc finger domain. Northern blot analysis demonstrated that ZNF189 was expressed in all tissues and cell types investigated, with the different splice variants showing tissue- or cell type-restricted expression patterns. This expression analysis also detected 2 additional unidentified splice variants. Odeberg et al. (1998) stated that the ZNF189 gene is well-conserved in several mammalian species.
Odeberg et al. (1998) determined that the ZNF189 gene spans approximately 11 kb and contains at least 4 exons.
By fluorescence in situ hybridization, Odeberg et al. (1998) mapped the ZNF189 gene to 9q22-q31.
Odeberg, J., Rosok, O., Gudmundsson, G. H., Ahmadian, A., Roshani, L., Williams, C., Larsson, C., Ponten, F., Uhlen, M., Asheim, H.-C., Lundeberg, J. Cloning and characterization of ZNF189, a novel human Kruppel-like zinc finger gene localized to chromosome 9q22-q31. Genomics 50: 213-221, 1998. [PubMed: 9653648] [Full Text: https://doi.org/10.1006/geno.1998.5309]