Entry - *603246 - GENERAL TRANSCRIPTION FACTOR 3C, POLYPEPTIDE 1; GTF3C1 - OMIM
 
* 603246

GENERAL TRANSCRIPTION FACTOR 3C, POLYPEPTIDE 1; GTF3C1


Alternative titles; symbols

TRANSCRIPTION FACTOR IIIC, ALPHA SUBUNIT
TFIIIC-ALPHA
TF3C-ALPHA
TFIIIC, 220-KD SUBUNIT


HGNC Approved Gene Symbol: GTF3C1

Cytogenetic location: 16p12.1   Genomic coordinates (GRCh38) : 16:27,460,613-27,549,913 (from NCBI)


TEXT

Description

Transcription factor IIIC (TF3C or GTF3C), a component of RNA polymerase III, is a multisubunit complex that binds B box promoter elements (summary by Lagna et al., 1994).


Cloning and Expression

Lagna et al. (1994) used oligonucleotides based on protein microsequencing results to clone rat GTF3C1 and a partial human GTF3C1 cDNA.

L'Etoile et al. (1994) cloned a full-length human cDNA encoding the approximately 230-kD TF3C-alpha subunit. The cDNA encodes a 2,109-amino acid polypeptide. Though functionally similar, the yeast and human TF3C-alpha subunit proteins have little sequence homology.


Gene Function

By use of immunodepletion assays, Lagna et al. (1994) showed that TF3C-alpha is absolutely required for RNA polymerase III transcription of 5S RNA (180420), tRNA, and VAI RNA genes.

L'Etoile et al. (1994) showed that TF3C-alpha binds to the box B promoter element.


Mapping

Gross (2014) mapped the GTF3C1 gene to chromosome 16p12.1 based on an alignment of the GTF3C1 sequence (GenBank BC137229) with the genomic sequence (GRCh37).


REFERENCES

  1. Gross, M. B. Personal Communication. Baltimore, Md. 6/27/2014.

  2. L'Etoile, N. D., Fahnestock, M. L., Shen, Y., Aebersold, R., Berk, A. J. Human transcription factor IIIC box B binding subunit. Proc. Nat. Acad. Sci. 91: 1652-1656, 1994. [PubMed: 8127861, related citations] [Full Text]

  3. Lagna, G., Kovelman, R., Sukegawa, J., Roeder, R. G. Cloning and characterization of an evolutionarily divergent DNA-binding subunit of mammalian TFIIIC. Molec. Cell. Biol. 14: 3053-3064, 1994. [PubMed: 8164661, related citations] [Full Text]


Contributors:
Matthew B. Gross - updated : 06/27/2014
Creation Date:
Jennifer P. Macke : 11/2/1998
mgross : 06/27/2014
carol : 6/15/2012
mgross : 4/27/2000
mgross : 4/27/2000
carol : 3/22/1999
alopez : 11/2/1998

* 603246

GENERAL TRANSCRIPTION FACTOR 3C, POLYPEPTIDE 1; GTF3C1


Alternative titles; symbols

TRANSCRIPTION FACTOR IIIC, ALPHA SUBUNIT
TFIIIC-ALPHA
TF3C-ALPHA
TFIIIC, 220-KD SUBUNIT


HGNC Approved Gene Symbol: GTF3C1

Cytogenetic location: 16p12.1   Genomic coordinates (GRCh38) : 16:27,460,613-27,549,913 (from NCBI)


TEXT

Description

Transcription factor IIIC (TF3C or GTF3C), a component of RNA polymerase III, is a multisubunit complex that binds B box promoter elements (summary by Lagna et al., 1994).


Cloning and Expression

Lagna et al. (1994) used oligonucleotides based on protein microsequencing results to clone rat GTF3C1 and a partial human GTF3C1 cDNA.

L'Etoile et al. (1994) cloned a full-length human cDNA encoding the approximately 230-kD TF3C-alpha subunit. The cDNA encodes a 2,109-amino acid polypeptide. Though functionally similar, the yeast and human TF3C-alpha subunit proteins have little sequence homology.


Gene Function

By use of immunodepletion assays, Lagna et al. (1994) showed that TF3C-alpha is absolutely required for RNA polymerase III transcription of 5S RNA (180420), tRNA, and VAI RNA genes.

L'Etoile et al. (1994) showed that TF3C-alpha binds to the box B promoter element.


Mapping

Gross (2014) mapped the GTF3C1 gene to chromosome 16p12.1 based on an alignment of the GTF3C1 sequence (GenBank BC137229) with the genomic sequence (GRCh37).


REFERENCES

  1. Gross, M. B. Personal Communication. Baltimore, Md. 6/27/2014.

  2. L'Etoile, N. D., Fahnestock, M. L., Shen, Y., Aebersold, R., Berk, A. J. Human transcription factor IIIC box B binding subunit. Proc. Nat. Acad. Sci. 91: 1652-1656, 1994. [PubMed: 8127861] [Full Text: https://doi.org/10.1073/pnas.91.5.1652]

  3. Lagna, G., Kovelman, R., Sukegawa, J., Roeder, R. G. Cloning and characterization of an evolutionarily divergent DNA-binding subunit of mammalian TFIIIC. Molec. Cell. Biol. 14: 3053-3064, 1994. [PubMed: 8164661] [Full Text: https://doi.org/10.1128/mcb.14.5.3053-3064.1994]


Contributors:
Matthew B. Gross - updated : 06/27/2014

Creation Date:
Jennifer P. Macke : 11/2/1998

Edit History:
mgross : 06/27/2014
carol : 6/15/2012
mgross : 4/27/2000
mgross : 4/27/2000
carol : 3/22/1999
alopez : 11/2/1998