Alternative titles; symbols
HGNC Approved Gene Symbol: BUD31
Cytogenetic location: 7q22.1 Genomic coordinates (GRCh38) : 7:99,408,969-99,419,616 (from NCBI)
The Xenopus G10 mRNA is a maternal transcript that is translationally activated during oocyte maturation. Using a PCR-subtractive hybridization strategy, Hla et al. (1995) isolated cDNAs that were induced by phorbol myristic acetate in human umbilical vein endothelial cells. One cDNA, designated EDG2, encoded a predicted 144-amino acid protein with 93% sequence identity to the predicted G10 protein. Both proteins contained an N-terminal acidic domain and a cysteine-rich C-terminal domain containing a potential C2C2-type zinc finger motif. The in vitro translated human EDG2 protein had a molecular mass of approximately 18 kD. RT-PCR analysis indicated that EDG2 is expressed ubiquitously.
Hsu et al. (2015) discovered that the spliceosome is a target of oncogenic stress in MYC (190080)-driven cancers. They identified BUD31 as a MYC-synthetic lethal gene in human mammary epithelial cells, and demonstrated that BUD31 is a component of the core spliceosome required for its assembly and catalytic activity. Core spliceosomal factors associated with BUD31 such as SF3B1 (605590) and U2AF1 (191317) are also required to tolerate oncogenic MYC. Notably, MYC hyperactivation induces an increase in total precursor mRNA synthesis, suggesting an increased burden on the core spliceosome to process pre-mRNA. In contrast to normal cells, partial inhibition of the spliceosome in MYC-hyperactivated cells leads to global intron retention, widespread defects in pre-mRNA maturation, and deregulation of many essential cell processes. Notably, genetic or pharmacologic inhibition of the spliceosome in vivo impairs survival, tumorigenicity, and metastatic proclivity of MYC-dependent breast cancers. Hsu et al. (2015) concluded that oncogenic MYC confers a collateral stress on splicing, and that components of the spliceosome may be therapeutic entry points for aggressive MYC-driven cancers.
Gross (2014) mapped the BUD31 gene to chromosome 7q22.1 based on an alignment of the BUD31 sequence (GenBank BC022821) with the genomic sequence (GRCh37).
Gross, M. B. Personal Communication. Baltimore, Md. 6/18/2014.
Hla, T., Jackson, A. Q., Appleby, S. B., Maciag, T. Characterization of edg-2, a human homologue of the Xenopus maternal transcript G10 from endothelial cells. Biochim. Biophys. Acta 1260: 227-229, 1995. [PubMed: 7841202] [Full Text: https://doi.org/10.1016/0167-4781(94)00219-s]
Hsu, T. Y.-T., Simon, L. M., Neill, N. J., Marcotte, R., Sayad, A., Bland, C. S., Echeverria, G. V., Sun, T., Kurley, S. J., Tyagi, S., Karlin, K. L., Dominguez-Vidana, R., and 17 others. The spliceosome is a therapeutic vulnerability in MYC-driven cancer. Nature 525: 384-388, 2015. [PubMed: 26331541] [Full Text: https://doi.org/10.1038/nature14985]