HGNC Approved Gene Symbol: SNRPG
Cytogenetic location: 2p13.3 Genomic coordinates (GRCh38) : 2:70,281,362-70,293,740 (from NCBI)
The 4 major small nuclear ribonucleoprotein particles (snRNPs), U1, U2, U4/U6, and U5, are essential components of the eukaryotic pre-mRNA splicing machinery. These snRNPs share 8 proteins which form the snRNP structural core: B/B-prime (SNRPB; 182282), D1 (SNRPD1; 601063), D2 (SNRPD2; 601061), D3 (SNRPD3; 601062), E (SNRPE; 128260), F (SNRPF; 603541), and G (SNRPG). These common proteins play an essential role in the biogenesis of the snRNPs. The assembly of the common proteins onto the small nuclear RNA (snRNA) appears to occur in at least 2 steps and involves the formation of RNA-free protein heterooligomers. The common proteins are referred to as Sm proteins because they represent the major targets for the anti-Sm autoantibodies which are diagnostic for SLE (152700) (summary by Hermann et al., 1995).
SNRPG migrates as a doublet of approximately 9 kD by high-TEMED SDS-PAGE. By performing RACE on a HeLa cell cDNA library using primers based on the partial protein sequence of SNRPG, Hermann et al. (1995) cloned SNRPG cDNAs. The predicted 76-amino acid protein contains 34% hydrophobic residues, most of which are located in the C-terminal half. The authors found that G protein translated in vitro from a single SNRPG mRNA also migrated as a doublet by high-TEMED SDS-PAGE. They suggested that the 2 bands represent conformational isomers of the same protein. Northern blot analysis revealed that the SNRPG gene is expressed as an approximately 0.5-kb mRNA in HeLa cells.
Hermann, H., Fabrizio, P., Raker, V. A., Foulaki, K., Hornig, H., Brahms, H., Luhrmann, R. snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions. EMBO J. 14: 2076-2088, 1995. [PubMed: 7744013] [Full Text: https://doi.org/10.1002/j.1460-2075.1995.tb07199.x]