Alternative titles; symbols
HGNC Approved Gene Symbol: RPS6KA5
Cytogenetic location: 14q32.11 Genomic coordinates (GRCh38) : 14:90,847,861-91,060,641 (from NCBI)
By searching an EST database with the sequence of the MAPKAP-K1 (see RPS6KA1, 601684) N-terminal kinase domain, Deak et al. (1998) identified cDNAs encoding 2 novel kinases: mitogen- and stress-activated protein kinase-1 (MSK1) and mitogen- and stress-activated protein kinase-2 (603606). The predicted 802-amino acid MSK1 protein contains 2 protein kinase domains, each of which includes the 11 subdomains characteristic of all protein kinases. MSK1 shares 43% protein sequence identity with the MAPKAP-K1 isoforms. Northern blot analysis indicated that MSK1 was expressed as a 4-kb mRNA in all tissues tested, with the highest levels of expression in brain, muscle, and placenta. Immunoelectron microscopy localized MSK1 to the nucleus.
Deak et al. (1998) found that MSK1 was activated in vitro and in vivo by either ERK or SAPK2 proteins. They presented evidence suggesting that MSK1, rather than MAPKAP-K1 or MAPKAP-K2/K3, mediates activation of the cAMP response element-binding protein (see CREB1, 123810) and activating transcription factor-1 (123803) by either growth factors or stress signals.
By radiation hybrid analysis, Jiang et al. (1999) mapped the RPS6KA5 gene to chromosome 14q31-q32.
Deak, M., Clifton, A. D., Lucocq, J. M., Alessi, D. R. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 17: 4426-4441, 1998. [PubMed: 9687510] [Full Text: https://doi.org/10.1093/emboj/17.15.4426]
Jiang, C., Yu, L., Tu, Q., Zhao, Y., Zhang, H., Zhao, S. Assignment of a member of the ribosomal protein S6 kinase family, RPS6KA5, to human chromosome 14q31-q32.1 by radiation hybrid mapping. Cytogenet. Cell Genet. 87: 261-261, 1999. [PubMed: 10702687] [Full Text: https://doi.org/10.1159/000015441]