HGNC Approved Gene Symbol: ZNF43
Cytogenetic location: 19p12 Genomic coordinates (GRCh38) : 19:21,804,946-21,852,065 (from NCBI)
Zinc finger proteins (ZNFs), which bind nucleic acids, perform many key functions, the most important of which is regulating transcription. See ZNF91 (603971) for general information on ZNFs.
By screening a B-cell cDNA library with an oligonucleotide corresponding to a sequence conserved in the zinc finger domains of C2H2 ZNFs, followed by screening a T-cell cDNA library, Lovering and Trowsdale (1991) isolated 2 forms of human ZNF43 cDNA which differ at the 5-prime end and in the 3-prime untranslated region. These cDNAs encode predicted 803- and 809-amino acid proteins containing N-terminal KRAB A and KRAB B boxes, 22 contiguous zinc finger domains of the C2H2 type, and a C-terminal region of 18 amino acids. Northern blot analysis showed that ZNF43 expression is low and limited to B- and T-cell lines.
By screening a cDNA library derived from a human undifferentiated embryonal carcinoma cell line with the coding sequence of the ZNF85 (603899) N-terminal nonfinger region, which spans the KRAB domain, Bellefroid et al. (1991) isolated cDNAs encoding 11 distinct KRAB ZNFs, including ZNF43, which they called HTF6. Lovering and Trowsdale (1991) noted that this HTF6 cDNA, which differs from the 2 ZNF43 cDNAs that they isolated, encodes a ZNF lacking the KRAB A box.
Bellefroid et al. (1993) reported the characterization of a subgroup of KRAB ZNFs, named the ZNF91 family, of which ZNF43 is a member. These proteins contain a conserved linker region, called the ZNF91-related spacer region, between their N-terminal KRAB domain and their first finger unit. The authors found that members of the ZNF91 family are widely expressed in human tissues, with the highest expression in T-lymphoid cells.
By somatic cell hybrid analysis and FISH, Bellefroid et al. (1993) mapped the ZNF43 gene to 19p13.1-p12.
Bellefroid, E. J., Marine, J. C., Ried, T., Lecocq, P. J., Riviere, M., Amemiya, C., Poncelet, D. A., Coulie, P. G., de Jong, P., Szpirer, C., Ward, D. C., Martial, J. A. Clustered organization of homologous KRAB zinc-finger genes with enhanced expression in human T lymphoid cells. EMBO J. 12: 1363-1374, 1993. [PubMed: 8467795] [Full Text: https://doi.org/10.1002/j.1460-2075.1993.tb05781.x]
Bellefroid, E. J., Poncelet, D. A., Lecocq, P. J., Revelant, O., Martial, J. A. The evolutionarily conserved Kruppel-associated box domain defines a subfamily of eukaryotic multifingered proteins. Proc. Nat. Acad. Sci. 88: 3608-3612, 1991. [PubMed: 2023909] [Full Text: https://doi.org/10.1073/pnas.88.9.3608]
Lovering, R., Trowsdale, J. A gene encoding 22 highly related zinc fingers is expressed in lymphoid cell lines. Nucleic Acids Res. 19: 2921-2928, 1991. [PubMed: 1711675] [Full Text: https://doi.org/10.1093/nar/19.11.2921]