Alternative titles; symbols
HGNC Approved Gene Symbol: POLR2J
Cytogenetic location: 7q22.1 Genomic coordinates (GRCh38) : 7:102,473,128-102,478,922 (from NCBI)
The S. cerevisiae RPB11 protein is 1 component of the multisubunit eukaryotic RNA polymerase II complex. For general information on the structure and function of RNA polymerase II, see 180660.
Using differential display PCR to identify mRNAs downregulated in colon carcinoma cell lines treated with the chemotherapeutic agent doxorubicin, Fanciulli et al. (1996) isolated cDNAs encoding a human homolog of RPB11. The predicted 117-amino acid human protein is 52% identical to yeast RPB11. Human RPB11 contains a conserved 18-amino acid region that is present in other RNA polymerase subunits and which may be involved in interactions among the subunits during assembly of the complex. Northern blot analysis revealed that the 0.6-kb RPB11 transcript is expressed ubiquitously, with the highest levels in heart and skeletal muscle.
Fanciulli et al. (1996) stated that RPB11 is the same gene identified by Pati (1994) as RPB14. However, Fanciulli et al. (1996) noted that the C-terminal regions of RPB11 and RPB14 are different, perhaps due to alternative splicing.
By Northern blot analysis, Grandemange et al. (2001) detected a 0.85-kb RPB11A transcript expressed at highest levels in heart and skeletal muscle.
Fanciulli et al. (1996) determined that doxorubicin treatment of cell lines reduced expression of RPB11 at both the mRNA and protein levels. They suggested that this drug may specifically inhibit transcriptional activity in vivo.
Using protein pull-down assays, Grandemange et al. (2001) determined that recombinant RPB11A interacted with RPB3 (POLR2C; 180663) but not with any of the 11 other human RNA polymerase II subunits examined.
Grandemange et al. (2001) determined that the POLR2J gene contains 4 exons.
By genomic sequence analysis, Grandemange et al. (2001) identified 3 possible loci on chromosome 7 for the POLR2J gene: 7p12, 7q11.23, and 7q22. They mapped the mouse Polr2j gene to a region of chromosome 5G that shares homology of synteny to human 7q11.23.
The International Radiation Hybrid Mapping Consortium mapped the POLR2J gene to chromosome 7q22-q31.1 (sts-L37127).
Fanciulli, M., Bruno, T., Cerboni, C., Bonetto, F., Iacobini, C., Frati, L., Piccoli, M., Floridi, A., Santoni, A., Punturieri, A. Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: new potential mechanisms of drug related toxicity. FEBS Lett. 384: 48-52, 1996. [PubMed: 8797801] [Full Text: https://doi.org/10.1016/0014-5793(96)00277-3]
Grandemange, S., Schaller, S., Yamano, S., Du Manoir, S., Shpakovski, G. V., Mattei, M.-G., Kedinger, C., Vigneron, M. A human RNA polymerase II subunit is encoded by a recently generated multigene family. BMC Molec. Biol. 2: 14, 2001. Note: Electronic Article. [PubMed: 11747469] [Full Text: https://doi.org/10.1186/1471-2199-2-14]
Pati, U. K. Human RNA polymerase II subunit hRPB14 is homologous to yeast RNA polymerase I, II, and III subunits (AC19 and RPB11) and is similar to a portion of the bacterial RNA polymerase alpha subunit. Gene 145: 289-292, 1994. [PubMed: 8056345] [Full Text: https://doi.org/10.1016/0378-1119(94)90022-1]