Alternative titles; symbols
HGNC Approved Gene Symbol: ITGBL1
Cytogenetic location: 13q33.1 Genomic coordinates (GRCh38) : 13:101,452,675-101,720,856 (from NCBI)
By screening an EST cDNA database for sequence homology with integrins (see 147557), Berg et al. (1999) identified overlapping partial-length cDNA clones from human osteoclastoma, fetal lung, and umbilical vein endothelial cDNA libraries. A subsequent screening identified a potential full-length clone from an osteoblast cell cDNA library. The novel cDNA clone was found to encode a 494-amino acid protein, which the authors designated TIED (ten beta-integrin EGF-like repeat domains). The sequence comprised a typical signal peptide, followed by a hydrophilic 471-amino acid domain containing 10 tandem EGF-like repeats strikingly similar to those found in the cysteine-rich 'stalk-like' structure of integrin-beta subunits. Northern blot analysis revealed a single RNA transcript of approximately 2.8 kb. TIED was widely expressed in many tissues, but readily detectable only in aorta.
By fluorescence in situ hybridization, Berg et al. (1999) mapped the ITGBL1 gene to human chromosome 13q33.
Berg, R. W., Leung, E., Gough, S., Morris, C., Yao, W.-P., Wang, S.-X., Ni, J., Krissansen, G. W. Cloning and characterization of a novel beta integrin-related cDNA coding for the protein TIED ('ten beta integrin EGF-like repeat domains') that maps to chromosome band 13q33: a divergent stand-alone integrin stalk structure. Genomics 56: 169-178, 1999. [PubMed: 10051402] [Full Text: https://doi.org/10.1006/geno.1998.5707]