Alternative titles; symbols
HGNC Approved Gene Symbol: CLCA3P
Cytogenetic location: 1p22.3 Genomic coordinates (GRCh38) : 1:86,634,276-86,655,376 (from NCBI)
CLCA3P belongs to the CLCA family of calcium-activated chloride channels. CLCA3P is expressed in various tissues, but unlike other CLCA proteins, it does not encode a functional transmembrane protein (Pauli et al., 2000).
By screening a human spleen cDNA library with a Lu-ECAM-1 cDNA and by RACE, Gruber and Pauli (1999) isolated a 3.6-kb cDNA encoding CLCA3. The CLCA3 cDNA is similar in length and sequence to the cDNAs of previously cloned family members. However, unlike all previously known CLCA cDNAs, the CLCA3 cDNA does not have a long open reading frame (ORF), but instead contains 2 smaller ORFs. The authors demonstrated that only 1 of these ORFs is of biologic significance and that it is expressed in mammalian cells as a secreted 37-kD glycoprotein. The deduced 262-amino acid CLCA3 protein corresponds to the N-terminal extracellular domain of other family members. RT-PCR detected CLCA3 expression in all human tissues examined. Gruber and Pauli (1999) verified the sequence of the spleen CLCA3 cDNA by isolating and sequencing a CLCA3 cDNA from human trachea. They concluded that CLCA3 is a structurally divergent member of the CLCA family and that it does not function as a channel protein.
By radiation hybrid analysis, Gruber and Pauli (1999) determined that the CLCA2 and CLCA3 genes map to 1p31-p22, where the CLCA1 gene had been assigned. Thus, all human CLCA family members known to that time were shown to be clustered on the short arm of chromosome 1 despite their moderately low levels of sequence homology and their heterogeneous expression patterns.
Pauli et al. (2000) reported that CLCA3 maps to a cluster of CLCA genes on chromosome 1p31-p22. The mouse Clca genes map to a syntenic gene cluster on chromosome 3H2-H3.
Gross (2016) mapped the CLCA3P pseudogene to chromosome 1p22.3 based on an alignment of the CLCA3P sequence (GenBank AF043976) with the genomic sequence (GRCh38).
Gross, M. B. Personal Communication. Baltimore, Md. 3/11/2016.
Gruber, A. D., Pauli, B. U. Clustering of the human CLCA gene family on the short arm of chromosome 1 (1p22-31). Genome 42: 1030-1032, 1999. [PubMed: 10584316] [Full Text: https://doi.org/10.1139/g99-006]
Gruber, A. D., Pauli, B. U. Molecular cloning and biochemical characterization of a truncated, secreted member of the human family of Ca(2+)-activated Cl- channels. Biochim. Biophys. Acta 1444: 418-423, 1999. [PubMed: 10095065] [Full Text: https://doi.org/10.1016/s0167-4781(99)00008-1]
Pauli, B. U., Abdel-Ghany, M., Cheng, H.-C., Gruber, A. D., Archibald, H. A., Elble, R. C. Molecular characteristics and functional diversity of CLCA family members. Clin. Exp. Pharm. Physiol. 27: 901-905, 2000. [PubMed: 11071307] [Full Text: https://doi.org/10.1046/j.1440-1681.2000.03358.x]