HGNC Approved Gene Symbol: HSBP1
Cytogenetic location: 16q23.3 Genomic coordinates (GRCh38) : 16:83,807,978-83,819,737 (from NCBI)
In response to stress, heat-shock factor-1 (HSF1; 140580) rapidly acquires DNA-binding activity while undergoing conformational transition from an inert non-DNA-binding monomer to active functional trimers. An inducible transcriptional response occurs during heat shock or upon recovery in nonstress conditions and involves dissociation of the HSF1 trimer and loss of activity. Using the hydrophobic repeats of the HSF1 trimerization domain as bait in a yeast 2-hybrid screen of a Madin-Darby canine kidney (MDCK) cDNA library, Satyal et al. (1998) identified canine heat-shock factor-binding protein-1 (HSBP1), which was used to probe a human cDNA library. They obtained a human HSBP1 cDNA encoding an acidic 76-amino acid protein that differs at only 2 positions from the canine protein. HSBP1 contains 2 potential hydrophobic repeats, which are essential for the interaction of HSF1 and HSBP1. HSBP1 interacts with HSF1 timers and negatively regulates HSF1 activity. Immunofluorescence staining revealed that HSBP1 is located predominantly in the nucleus. Northern blot analysis revealed that HSBP1 is expressed in HeLa, COS-7, 3T3, and MDCK cell lines unaffected by heat shock and in all animal tissues tested. HSBP1 also interacts with Hsp70, but only during the attenuation phase of the heat-shock response when HSF1 DNA-binding activity is lost.
Gross (2014) mapped the HSBP1 gene to chromosome 16q23.3 based on an alignment of the HSBP1 sequence (GenBank AF068754) with the genomic sequence (GRCh37).
Gross, M. B. Personal Communication. Baltimore, Md. 3/24/2014.
Satyal, S. H., Chen, D., Fox, S. G., Kramer, J. M., Morimoto, R. I. Negative regulation of the heat shock transcriptional response by HSBP1. Genes Dev. 12: 1962-1974, 1998. [PubMed: 9649501] [Full Text: https://doi.org/10.1101/gad.12.13.1962]