Alternative titles; symbols
HGNC Approved Gene Symbol: AKAP8
Cytogenetic location: 19p13.12 Genomic coordinates (GRCh38) : 19:15,353,385-15,379,787 (from NCBI)
A-kinase anchor proteins (AKAPs; see 602449) direct activity of protein kinase A (PKA; see 176911) by tethering the enzyme near its physiologic substrates.
By screening a human cerebellum cDNA library with rat AKAP95 as a probe, Eide et al. (1998) obtained a cDNA encoding a novel AKAP protein, AKAP8, which the authors designated AKAP95. The deduced AKAP8 protein contains 692 amino acids and shows 89% sequence identity to rat AKAP95. Sequence analysis predicted that the PKA RII-binding region comprises residues 572 to 589 and that a nuclear localization sequence of basic residues comprises residues 368 to 377. Northern blot analysis revealed expression of 4.3- and 5.0-kb transcripts at high levels in heart, liver, skeletal muscle, liver, kidney, and pancreas; low or no expression was detected in brain, placenta, and lung. Immunoprecipitation and immunofluorescence microscopy analyses showed that AKAP8 is located in the nucleus during interphase, whereas RII is excluded from the nucleus. During mitosis, AKAP8 is localized outside the metaphase plate and overlaps with RII. The proteins start to segregate in anaphase and are separated as AKAP8 reenters the nucleus in telophase, indicating a cell cycle-dependent interaction between AKAP8 and RII PKA.
By somatic cell hybrid analysis, Eide et al. (1998) mapped the AKAP8 gene to chromosome 19p13.1-q12.
Eide, T., Coghlan, V., Orstavik, S., Holsve, C., Solberg, R., Skalhegg, B. S., Lamb, N. J. C., Langeberg, L., Fernandez, A., Scott, J. D., Jahnsen, T., Tasken, K. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Exp. Cell Res. 238: 305-316, 1998. [PubMed: 9473338] [Full Text: https://doi.org/10.1006/excr.1997.3855]