Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF235
Cytogenetic location: 19q13.31 Genomic coordinates (GRCh38) : 19:44,286,348-44,305,025 (from NCBI)
By screening a human monoblast cell line (U-937) cDNA library with a degenerate oligonucleotide based on a conserved region of Kruppel-related zinc finger proteins that encompasses the H/C link, Abrink et al. (1995) isolated cDNAs encoding 42 distinct Kruppel-related zinc finger proteins, including ZFP93, which they called HZF6. The partial ZFP93 cDNA, which lacks 5-prime and 3-prime coding sequence, encodes at least 7 zinc finger motifs. Northern blot analysis detected a major 5.5-kb ZFP93 transcript in HeLa cells, U-937 cells, and PMA-induced U-937 cells, with higher expression in the monocyte/macrophage lineages.
Shannon and Stubbs (1998) isolated cDNAs representing a full-length ZFP93 transcript. The predicted 738-amino acid ZFP93 protein contains a KRAB A domain very near the N terminus, an adjacent KRAB B domain, a spacer region, and 15 tandemly arrayed C2H2-type zinc fingers. Northern blot analysis of adult human tissues detected a 3.1-kb ZFP93 transcript that was widely expressed, including in spleen, thymus, blood leukocytes, liver, pancreas, small intestine, colon, kidney, heart, brain, skeletal muscle, testis, prostate, ovary, and placenta.
Zinc finger proteins have been shown to interact with nucleic acids and to have diverse functions, such as stimulation of transcription, transcriptional repression, binding to single-stranded DNA, binding to RNA, or bifunctional DNA- and RNA-binding activities. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc. Zinc fingers bind divalent zinc ions by 1 of at least 5 different combinations of cysteines and histidines, including the eukaryotic C2H2, C4, and C6 motifs. The Drosophila protein Kruppel belongs to a very large family of related C2H2 zinc finger proteins in which the zinc finger motifs are located in smaller or larger tandem arrays. The spacer region connecting successive zinc finger repeats, known as the H/C link, is highly conserved among members of this family, showing a higher degree of homology than the finger motif. Structurally conserved motifs outside the zinc finger regions subdivide the Kruppel family into smaller subfamilies. One such motif is the Kruppel-associated box (KRAB), which is an approximately 75-amino acid domain that is usually positioned in the N terminus of the protein and which may form a leucine zipper-like structure (summary by Abrink et al., 1995).
By PCR, Shannon and Stubbs (1998) localized the ZFP93 gene to a specific cosmid within a 19q13.2 cosmid contig.
Shannon et al. (1996) mapped the homologous mouse Zfp93 gene within a cluster of KRAB-containing zinc finger genes near the Xrcc1 gene on chromosome 7.
Abrink, M., Aveskogh, M., Hellman, L. Isolation of cDNA clones for 42 different Kruppel-related zinc finger proteins expressed in the human monoblast cell line U-937. DNA Cell Biol. 14: 125-136, 1995. [PubMed: 7865130] [Full Text: https://doi.org/10.1089/dna.1995.14.125]
Shannon, M., Ashworth, L. K., Mucenski, M. L., Lamerdin, J. E., Branscomb, E., Stubbs, L. Comparative analysis of a conserved zinc finger gene cluster on human chromosome 19q and mouse chromosome 7. Genomics 33: 112-120, 1996. [PubMed: 8617494] [Full Text: https://doi.org/10.1006/geno.1996.0166]
Shannon, M., Stubbs, L. Analysis of homologous XRCC1-linked zinc-finger gene families in human and mouse: evidence for orthologous genes. Genomics 49: 112-121, 1998. [PubMed: 9570955] [Full Text: https://doi.org/10.1006/geno.1998.5230]