Alternative titles; symbols
HGNC Approved Gene Symbol: TAF1A
Cytogenetic location: 1q41 Genomic coordinates (GRCh38) : 1:222,554,105-222,589,933 (from NCBI)
The TAF1A gene encodes a TATA box-binding protein (TBP; 600075)-associated factor (TAF) present in the multisubunit SL1 complex, which is required for RNA polymerase I to synthesize ribosomal RNA. SL1 consists of TBP, which is a universal GTF for all 3 RNA polymerases, and 3 TAFs (summary by Di Pietro et al., 2000).
Using immunoaffinity chromatography with anti-TBP, followed by SDS-PAGE analysis, Comai et al. (1994) isolated the purified 48-kD subunit of SL1. By screening a human cDNA library using guessmer probes corresponding to peptide sequences of the 48-kD subunit of SL1, they obtained a cDNA encoding TAF1A, which they called TAFI48. Comai et al. (1994) also obtained cDNAs encoding TAF1B (604904) and TAF1C (604905). TAF1A encodes a deduced 450-amino acid protein. Western blot analysis confirmed that TAF1A is expressed as a 48-kD protein. Analysis of SL1 subunit interactions showed that all 3 TAF1 proteins bind to TBP and to each other. However, binding of the SL1 complex to TBP excluded binding of the RNA polymerase II transcription factor TFIID (see TAF2A; 313650) to TBP. Comai et al. (1994) concluded that this mutually exclusive binding directs the formation of promoter- and RNA polymerase-selective TBP-TAF complexes.
The International Radiation Hybrid Mapping Consortium mapped the TAF1A gene to 1q (L39060). By fluorescence in situ hybridization, Di Pietro et al. (2000) mapped the TAF1A gene to chromosome 1q42.
Comai, L., Zomerdijk, J. C. B. M., Beckmann, H., Zhou, S., Admon, A., Tjian, R. Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits. Science 266: 1966-1972, 1994. [PubMed: 7801123] [Full Text: https://doi.org/10.1126/science.7801123]
Di Pietro, C., Rapisarda, A., Amico, V., Bonaiuto, C., Viola, A., Scalia, M., Motta, S., Amato, A., Engel, H., Messina, A., Sichel, G., Grzeschik, K.-H., Purrello, M. Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAFI48, TAFI63 and TAFI110 subunits of class I general transcription initiation factor SL1. Cytogenet. Cell Genet. 89: 133-136, 2000. [PubMed: 10894955] [Full Text: https://doi.org/10.1159/000015592]