Alternative titles; symbols
HGNC Approved Gene Symbol: TAF1C
Cytogenetic location: 16q24.1 Genomic coordinates (GRCh38) : 16:84,177,855-84,187,057 (from NCBI)
The TAF1C gene encodes a TATA box-binding protein (TBP; 600075)-associated factor (TAF) present in the multisubunit SL1 complex, which is required for RNA polymerase I to synthesize ribosomal RNA. SL1 consists of TBP, which is a universal GTF for all 3 RNA polymerases, and 3 TAFs (summary by Di Pietro et al., 2000).
Using immunoaffinity chromatography with anti-TBP, followed by SDS-PAGE analysis, Comai et al. (1994) isolated the purified 110-kD subunit of SL1. By screening teratocarcinoma and HeLa cell cDNA libraries using degenerate PCR primers corresponding to peptide sequences of the 110-kD subunit of SL1, they obtained a cDNA encoding TAF1C, which they called TAFI110. Comai et al. (1994) also obtained cDNAs encoding TAF1A (604903) and TAF1B (604904). TAF1C encodes a deduced 869-amino acid protein. Western blot analysis confirmed that TAF1C is expressed as a 110-kD protein. Analysis of SL1 subunit interactions showed that all 3 TAF1 proteins bind to TBP and to each other. However, binding of the SL1 complex to TBP excluded binding of the RNA polymerase II transcription factor TFIID (see TAF2A; 313650) to TBP. Comai et al. (1994) concluded that this mutually exclusive binding directs the formation of promoter- and RNA polymerase-selective TBP-TAF complexes.
The International Radiation Hybrid Mapping Consortium mapped the TAF1C gene to 16q (L39059). Di Pietro et al. (2000) mapped the TAF1C gene to chromosome 16q24 by fluorescence in situ hybridization.
Comai, L., Zomerdijk, J. C. B. M., Beckmann, H., Zhou, S., Admon, A., Tjian, R. Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits. Science 266: 1966-1972, 1994. [PubMed: 7801123] [Full Text: https://doi.org/10.1126/science.7801123]
Di Pietro, C., Rapisarda, A., Amico, V., Bonaiuto, C., Viola, A., Scalia, M., Motta, S., Amato, A., Engel, H., Messina, A., Sichel, G., Grzeschik, K.-H., Purrello, M. Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAFI48, TAFI63 and TAFI110 subunits of class I general transcription initiation factor SL1. Cytogenet. Cell Genet. 89: 133-136, 2000. [PubMed: 10894955] [Full Text: https://doi.org/10.1159/000015592]