Alternative titles; symbols
HGNC Approved Gene Symbol: PHTF1
Cytogenetic location: 1p13.2 Genomic coordinates (GRCh38) : 1:113,696,831-113,759,888 (from NCBI)
Homeodomain proteins, such as PHTF1, are transcriptional regulators that control a variety of cell fates. The homeodomain is a 60-amino acid sequence containing a helix-turn-helix motif that recognizes and binds specific DNA sequences (summary by Manuel et al., 2000).
To identify genes regulated during hematopoietic differentiation, Raich et al. (1999) constructed a subtracted cDNA library using mRNAs isolated from human erythroleukemic cells cultured in the presence or absence of 12-O-tetradecanoylphorbol-13-acetate. They identified a cDNA encoding PHTF1, which they referred to as PHTF. The predicted 763-amino acid PHTF1 protein contains an atypical homeodomain.
By screening a mouse testis cDNA library, Manuel et al. (2000) cloned Phtf1 cDNAs with 2 different 5-prime UTRs, one of which has a CpG island. Database analysis revealed the same 2 UTRs in human and rat PHTF1 cDNAs. The deduced 762-amino acid mouse protein is 92% identical to human PHTF1. Database analysis identified a Drosophila ortholog of PHTF1. Highest identity across species occurs in a 110-amino acid N-terminal domain, the homeodomain, and a 110-amino acid C-terminal domain that includes a possible leucine zipper and a helix-loop-helix dimerization domain. All members of the PHTF family, including PHTF1, also contain a putative nuclear localization signal and possible sites for phosphorylation. Northern blot analysis detected variable expression of PHFT1 in human and mouse tissues, with highest expression in testis.
By in situ hybridization, Raich et al. (1999) mapped the PHTF1 gene to 1p13.
Manuel, A., Beaupain, D., Romeo, P. H., Raich, N. Molecular characterization of a novel gene family (PHTF) conserved from Drosophila to mammals. Genomics 64: 216-220, 2000. [PubMed: 10729229] [Full Text: https://doi.org/10.1006/geno.1999.6079]
Raich, N., Mattei, M. G., Romeo, P. H., Beaupain, D. PHTF, a novel atypical homeobox gene on chromosome 1p13, is evolutionarily conserved. Genomics 59: 108-109, 1999. [PubMed: 10395808] [Full Text: https://doi.org/10.1006/geno.1999.5836]