Alternative titles; symbols
HGNC Approved Gene Symbol: SNAPC2
Cytogenetic location: 19p13.2 Genomic coordinates (GRCh38) : 19:7,920,338-7,923,250 (from NCBI)
SNAPC2 encodes a subunit of the snRNA-activating protein complex (SNAPc), which is required for transcription of both RNA polymerase II and III snRNA genes (see SNAPC1, 600591) (Henry et al., 1995).
By SDS-PAGE and peptide analysis, Henry et al. (1995) showed that the SNAPC subunits, including SNAPC2, which they called SNAP45, are associated with TBP. Using a similar purification system and PCR analysis, Yoon and Roeder (1996) obtained cDNAs encoding SNAPC1 and SNAPC2, which they called PSE-binding transcription factor (PTF)-gamma and -delta, respectively. Sequence analysis predicted that the deduced 334-amino acid SNAPC2 is an acidic (pI of 5.7), proline-rich (13.8% of all residues) protein. Immunodepletion experiments showed that SNAPC2 is required for the transcription of both pol II- and pol III-dependent snRNA genes. Immunoblot analysis suggested that in the presence of high salt, the SNAPC subunits bind more strongly with each other than they do with TBP.
Sadowski et al. (1996) cloned SNAPC2 using PCR analysis. They noted that the proline heptad repeat motif of SNAPC2, which is identical to the one present in the C terminus of the large subunit of pol II, contains several potential phosphorylation sites that may account for the expressed protein being larger than the predicted 36 kD. EMSA analysis showed that SNAPC2 is part of the SNAPC-PSE complex.
Gross (2014) mapped the SNAPC2 gene to chromosome 19p13.2 based on an alignment of the SNAPC2 sequence (GenBank BC011868) with the genomic sequence (GRCh37).
Gross, M. B. Personal Communication. Baltimore, Md. 4/16/2014.
Henry, R. W., Sadowski, C. L., Kobayashi, R., Hernandez, N. A TBP-TAF complex required for transcription of human snRNA genes by RNA polymerases II and III. Nature 374: 653-656, 1995. [PubMed: 7715707] [Full Text: https://doi.org/10.1038/374653a0]
Sadowski, C. L., Henry, R. W., Kobayashi, R., Hernandez, N. The SNAP45 subunit of the small nuclear RNA (snRNA) activating protein complex is required for RNA polymerase II and III snRNA gene transcription and interacts with the TATA box binding protein. Proc. Nat. Acad. Sci. 93: 4289-4293, 1996. [PubMed: 8633057] [Full Text: https://doi.org/10.1073/pnas.93.9.4289]
Yoon, J.-B., Roeder, R. G. Cloning of two proximal sequence element-binding transcription factor subunits (gamma and delta) that are required for transcription of small nuclear RNA genes by RNA polymerases II and III and interact with the TATA-binding protein. Molec. Cell Biol. 16: 1-9, 1996. [PubMed: 8524284] [Full Text: https://doi.org/10.1128/MCB.16.1.1]