Alternative titles; symbols
HGNC Approved Gene Symbol: BTAF1
Cytogenetic location: 10q23.32 Genomic coordinates (GRCh38) : 10:91,923,770-92,031,437 (from NCBI)
Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.
By screening a B-cell cDNA library using an EST with homology to the C-terminal region of the yeast transcription factor Mot1 as the probe, followed by RT-PCR using HeLa cell mRNA and the isolation of a genomic fragment, van der Knaap et al. (1997) obtained a cDNA encoding TAFII170. Cofractionation and coprecipitation experiments showed that the protein encoded by the TAFII170 cDNA is the TAF subunit of B-TFIID. Sequence analysis predicted that the 1,849-amino acid TAFII170 protein harbors the 7 motifs characteristic for proteins with ATPase activity. The ATPase region of TAFII170 shares 62% amino acid identity with yeast Mot1. Whereas previous analysis indicated that TAFII170 migrates at 170 to 172 kD, van der Knaap et al. (1997) found that TAFII170 migrates closer to its calculated molecular mass of 206 kD. Immunoblot analysis indicated that recombinant TAF170 can associate with recombinant TBP.
By microsequence analysis of purified TAF172, followed by PCR, Chicca et al. (1998) obtained a cDNA encoding TAF172, which shares 37% amino acid identity and 50% similarity with yeast Mot1.
Using Western blot analysis, Chicca et al. (1998) determined that the stable association of TBP and TAF172 requires the presence of DNA. Electrophoretic mobility shift analysis showed that TAF172 possesses DNA-dependent ATPase activity that drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.
By genomic clone analysis, van der Knaap et al. (2000) determined that the TAFII170 gene spans approximately 130 kb and contains 37 introns.
By FISH and radiation hybrid analysis, van der Knaap et al. (2000) mapped the BTAF1 (TAFII170) gene to chromosome 10q22-q23.
Chicca, J. J., II, Auble, D. T., Pugh, B. F. Cloning and biochemical characterization of TAF-172, a human homolog of yeast Mot1. Molec. Cell. Biol. 18: 1701-1710, 1998. [PubMed: 9488487] [Full Text: https://doi.org/10.1128/MCB.18.3.1701]
van der Knaap, J. A., Borst, J. W., van der Vliet, P. C., Gentz, R., Timmers, H. Th. M. Cloning of the cDNA for the TATA-binding protein-associated factor(II)170 subunit of transcription factor B-TFIID reveals homology to global transcription regulators in yeast and Drosophila. Proc. Nat. Acad. Sci. 94: 11827-11832, 1997. [PubMed: 9342322] [Full Text: https://doi.org/10.1073/pnas.94.22.11827]
van der Knaap, J. A., van den Boom, V., Kuipers, J., van Eijk, M. J. T., van der Vliet, P. C., Timmers, H. Th. M. The gene for human TATA-binding-protein-associated factor (TAF-II) 170: structure, promoter and chromosomal localization. Biochem. J. 345: 521-527, 2000. [PubMed: 10642510] [Full Text: https://doi.org/10.1042/0264-6021:3450521]