Alternative titles; symbols
HGNC Approved Gene Symbol: PDS5B
Cytogenetic location: 13q13.1 Genomic coordinates (GRCh38) : 13:32,586,452-32,778,019 (from NCBI)
In the prostate of adult mammals, most epithelial cells are in a state of proliferative quiescence. Androgens regulate this effect by inducing cell cycle arrest in the G0/G1 phase. Geck et al. (2000) identified potential mediators of this androgen-induced proliferative shutoff by means of subtractive cDNA libraries. The expression pattern of one of these sequences, designated AS3, strongly correlated with the expression of the androgen-induced proliferative shutoff both temporally and dosewise. The AS3 gene is upregulated during androgen-induced proliferative shutoff and induces cell proliferation arrest when expressed in a retrovirus transduced model. The deduced 1,391-amino acid AS3 protein has putative transactivating features, protein-protein interaction motifs (coiled coil and leucine zipper), and DNA-binding domains, suggesting that AS3 is a transcription factor. AS3 also has a protein-kinase motif, suggesting that it may act by phosphorylating a target protein.
Geck et al. (1999) demonstrated that the transcript of the AS3 gene has 34 exons spanning approximately 200 kb of genomic DNA.
By database analysis, Geck et al. (1999) mapped the AS3 gene to chromosome 13q12-q13, downstream of the breast cancer susceptibility gene BRCA2 (600185) and centromeric to the retinoblastoma (RB1; 614041) locus.
Geck et al. (2001) presented data on the location of the AS3 gene in relation to BRCA2 and pointed out that the D13S171 marker, which had been widely used as an intragenic marker of BRCA2, is actually located in the center of the 200-kb AS3 gene. The microsatellite instability of the S171 marker links the AS3 gene to a variety of cancers.
Geck, P., Maffini, M. V., Szelei, J., Sonnenschein, C., Soto, A. M. Androgen-induced proliferative quiescence in prostate cancer cells: the role of AS3 as its mediator. Proc. Nat. Acad. Sci. 97: 10185-10190, 2000. [PubMed: 10963680] [Full Text: https://doi.org/10.1073/pnas.97.18.10185]
Geck, P., Sonnenschein, C., Soto, A. M. The D13S171 marker, misannotated to BRCA2, links the AS3 gene to various cancers. (Letter) Am. J. Hum. Genet. 69: 461-463, 2001. [PubMed: 11452363] [Full Text: https://doi.org/10.1086/321968]
Geck, P., Szelei, J., Jimenez, J., Sonnenschein, C., Soto, A. M. Early gene expression during androgen-induced inhibition of proliferation of prostate cancer cells: a new suppressor candidate on chromosome 13, in the BRCA2-Rb1 locus. J. Steroid Biochem. Molec. Biol. 68: 41-50, 1999. [PubMed: 10215036] [Full Text: https://doi.org/10.1016/s0960-0760(98)00165-4]