Alternative titles; symbols
HGNC Approved Gene Symbol: NFYC
Cytogenetic location: 1p34.2 Genomic coordinates (GRCh38) : 1:40,691,704-40,771,603 (from NCBI)
NFYC, together with NFYA (189903) and NFYB (189904), form the ubiquitous heterotrimeric protein nuclear factor Y (NFY), also termed CCAAT-binding factor (CBF). The CCAAT box is a widespread element found in promoters and enhancers (summary by Bellorini et al., 1997). NFYB and NFYC must interact and dimerize for NFYA association and DNA binding, most notably to Y-box elements such as CCAAT in major histocompatibility (MHC) class II promoters (see MHC2TA; 600005), to occur (Nakshatri et al., 1996).
Using the rat Nfyc sequence and PCR to screen HeLa cell cDNA libraries, Nakshatri et al. (1996) and Bellorini et al. (1997) isolated cDNAs encoding NFYC. By differential hybridization of a gridded fetal brain cDNA library, Dmitrenko et al. (1997) also cloned the NFYC gene, which they termed CBFC. Sequence analysis predicted that the 335-amino acid NFYC protein, which is approximately 97% identical to the rodent Nfyc sequences, contains a yeast Hap5 homology region (approximately 75% identity with the corresponding region in Hap5) and a glutamine-rich region. Northern blot analysis detected a major 2.3- and a minor 1.5-kb NFYC transcript in all tissues tested, with higher amounts of both mRNAs in testis (Bellorini et al., 1997).
Chang et al. (2008) showed that the NFYC gene has 10 exons and that the microRNAs MIRN30E and MIRN30C1 are contained within intron 5.
By interspecific backcross and segregation analysis, Sinha et al. (1996) mapped the mouse Nfyc gene to chromosome 4 in a region showing homology of synteny to human 1p32. Scott (2000) mapped the NFYC gene to 1p34.2-p33 based on sequence similarity between the NFYC sequence (GenBank D89986) and the chromosome 1 clone RP5-1066H13 (GenBank AL158843).
Bellorini, M., Zemzoumi, K., Farina, A., Berthelsen, J., Piaggio, G., Mantovani, R. Cloning and expression of human NF-YC. Gene 193: 119-125, 1997. [PubMed: 9249075] [Full Text: https://doi.org/10.1016/s0378-1119(97)00109-1]
Chang, T.-C., Yu, D., Lee, Y.-S., Wentzel, E. A., Arking, D. E., West, K. M., Dang, C. V., Thomas-Tikhonenko, A., Mendell, J. T. Widespread microRNA repression by Myc contributes to tumorigenesis. Nature Genet. 40: 43-50, 2008. [PubMed: 18066065] [Full Text: https://doi.org/10.1038/ng.2007.30]
Dmitrenko, V., Garifulin, O., Kavsan, V. Isolation and sequence analysis of the cDNA encoding subunit C of human CCAAT-binding transcription factor. Gene 197: 161-163, 1997. [PubMed: 9332362] [Full Text: https://doi.org/10.1016/s0378-1119(97)00255-2]
Nakshatri, H., Bhat-Nakshatri, P., Currie, R. A. Subunit association and DNA binding activity of the heterotrimeric transcription factor NF-Y is regulated by cellular redox. J. Biol. Chem. 271: 28784-28791, 1996. [PubMed: 8910521] [Full Text: https://doi.org/10.1074/jbc.271.46.28784]
Scott, A. F. Personal Communication. Baltimore, Md. 10/16/2000.
Sinha, S., Maity, S. N., Seldin, M. F., de Crombrugghe, B. Chromosomal assignment and tissue expression of CBF-C/NFY-C, the third subunit of the mammalian CCAAT-binding factor. Genomics 37: 260-263, 1996. [PubMed: 8921405] [Full Text: https://doi.org/10.1006/geno.1996.0555]