Alternative titles; symbols
HGNC Approved Gene Symbol: PBOV1
Cytogenetic location: 6q23.3 Genomic coordinates (GRCh38) : 6:138,215,986-138,218,491 (from NCBI)
By differential display analysis of normal and cancerous prostate tissue, An et al. (2000) isolated a cDNA encoding UROC28. Sequence analysis predicted that the 135-amino acid protein contains a transmembrane domain, 3 potential phosphorylation sites, and 1 potential myristoylation site. Northern blot analysis revealed expression of a 2.0-kb transcript in colon, prostate, small intestine, testis, and spleen, with lower expression in thymus, ovary, and peripheral blood leukocytes. A 2.4-kb transcript was also detected in prostate and spleen. RT-PCR analysis detected upregulated expression of UROC28 in prostate, breast, and bladder cancer, but not in lung and colon cancer, compared with normal tissue. In situ hybridization analysis showed preferential expression of UROC28 in prostate and breast glandular or ductal epithelial cells and elevated, heterogeneous expression of UROC28 in prostate and breast tumors. Immunohistochemical analysis detected primarily cytoplasmic as well as nuclear expression. Western blot blot analysis showed significantly increased serum expression of UROC28 in prostate cancer compared with controls.
Using FISH, An et al. (2000) mapped the UROC28 gene to 6q23-q24, a region associated with loss of androgen dependence in prostate tumors.
Using chromatin immunoprecipitation analysis, Yang et al. (2018) showed that C/EBP-beta (CEBPB; 189965) bound to the promoter of the long noncoding RNA NTT (602154) and regulated NTT expression in human THP-1 cells. NTT acted in cis on genes in its proximity and regulated their expression. In particular, NTT enhanced expression of the downstream gene PBOV1 by interacting with HNRNPU (602869), which also bound to 2 positions in the PBOV1 promoter region. RT-PCR analysis revealed that C/EBP-beta, NTT, and PBOV1 expression was highly elevated in peripheral blood mononuclear cells of untreated rheumatoid arthritis (RA; 180300) patients, and the higher expression levels appeared to be associated with higher disease inflammatory status. Overexpression of PBOV1 in THP-1 cells resulted in cell-cycle arrest at G1 phase and promoted differentiation towards macrophages and increased CXCL10 (147310) secretion.
An, G., Ng, A. Y., Meka, C. S. R., Luo, G., Bright, S. P., Cazares, L., Wright, G. L., Jr., Veltri, R. W. Cloning and characterization of UROC28, a novel gene overexpressed in prostate, breast, and bladder cancers. Cancer Res. 60: 7014-7020, 2000. [PubMed: 11156405]
Yang, C.-A., Li, J.-P., Yen, J.-C., Lai, I-L., Ho, Y.-C., Lan, J.-L., Chang, J.-G. lncRNA NTT/PBOV1 axis promotes monocyte differentiation and is elevated in rheumatoid arthritis. Int. J. Molec. Sci. 19: 2806, 2018. [PubMed: 30231487] [Full Text: https://doi.org/10.3390/ijms19092806]