Alternative titles; symbols
HGNC Approved Gene Symbol: BCAS2
Cytogenetic location: 1p13.2 Genomic coordinates (GRCh38) : 1:114,567,557-114,581,615 (from NCBI)
Using an 8-way (i.e., 8 cell lines) differential display analysis, followed by screening a fetal brain cDNA library, Nagasaki et al. (1999) isolated a cDNA encoding BCAS2, which they termed DAM1 (DNA amplified in mammary carcinoma-1). The deduced 225-amino acid protein contains a central potential N-glycosylation site and multiple N-terminal potential phosphorylation sites. Northern and Southern blot analyses revealed expression of a 1.5-kb transcript at various levels in multiple breast cancer cell lines, with greatest amplification in BT-20 and MCF-7 cells. BCAS2 was ubiquitously expressed in normal tissues.
Neubauer et al. (1998) reported the presence of a sequence identical to BCAS2 in a putative spliceosome-associated protein, which they termed SPF27.
Nagasaki et al. (1999) mapped the BCAS2 gene to 1p21-p13.3 by FISH. They stated that among the chromosomal alterations observed in breast cancer, a gain of 1q and loss of 1p regions are the most commonly observed.
Nagasaki, K., Maass, N., Manabe, T., Hanzawa, H., Tsukada, T., Kikuchi, K., Yamaguchi, K. Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines. Cancer Lett. 140: 219-226, 1999. [PubMed: 10403562] [Full Text: https://doi.org/10.1016/s0304-3835(99)00091-9]
Neubauer, G., King, A., Rappsilber, J., Calvio, C., Watson, M., Ajuh, P., Sleeman, J., Lamond, A., Mann, M. Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex. Nature Genet. 20: 46-50, 1998. [PubMed: 9731529] [Full Text: https://doi.org/10.1038/1700]