Alternative titles; symbols
HGNC Approved Gene Symbol: ZBTB32
Cytogenetic location: 19q13.12 Genomic coordinates (GRCh38) : 19:35,704,558-35,717,038 (from NCBI)
To identify FANCC (613899) binding partners that might shed light on the function of the FA pathway, Hoatlin et al. (1999) used a yeast 2-hybrid screen of a B-cell cDNA library with N-terminal sequences of FANCC as bait. By confirming the interactions using GST pull-down assays, followed by RT-PCR, Hoatlin et al. (1999) isolated a cDNA encoding FAZF. Sequence analysis predicted that the 487-amino acid protein, which is similar to PLZF (176797), contains an N-terminal POZ/BTB domain and 3 zinc fingers. Northern blot analysis detected weak expression of a 1.4-kb transcript in most tissues tested except peripheral blood leukocytes, where strong expression was detected, and testis, where strong expression of 2.0-, 2.7-, and 4.4-kb transcripts was detected. Coimmunoprecipitation analysis showed that FAZF associates with FANCC independent of the BTB/POZ domain. Indirect immunofluorescence and confocal microscopy demonstrated almost entirely nuclear expression for FAZF and colocalization with FANCC, which Hoatlin et al. (1999) confirmed is also expressed in cytoplasm in some cells; FANCC and PLZF did not colocalize. EMSA analysis suggested that PLZF and FAZF bind to CpG islands, interact with each other through the POZ domain of PLZF, and repress transcription by a similar mechanism.
By database searching, genomic analysis, RT-PCR, and yeast 2-hybrid screening, Lin et al. (1999) cloned FAZF, which they termed TZFP. Northern blot analysis detected expression of 3 transcripts only in testis.
Using genomic sequence analysis, Lin et al. (1999) determined that the TZFP gene contains 5 exons.
By genomic sequence analysis, Hoatlin et al. (1999) mapped the FAZF gene to 19q13.1.
Hoatlin, M. E., Zhi, Y., Ball, H., Silvery, K., Melnick, A., Stone, S., Arai, S., Hawe, N., Owen, G., Zelent, A., Licht, J. D. A novel BTB/POZ transcriptional repressor protein interacts with the Fanconi anemia group C protein and PLZF. Blood 94: 3737-3747, 1999. [PubMed: 10572087]
Lin, W., Lai, C.-H., Tang, C.-J. C., Huang, C.-J., Tang, T. K. Identification and gene structure of a novel human PLZF-related transcription factor gene, TZFP. Biochem. Biophys. Res. Commun. 264: 789-795, 1999. [PubMed: 10544010] [Full Text: https://doi.org/10.1006/bbrc.1999.1594]