Alternative titles; symbols
HGNC Approved Gene Symbol: SRRM1
Cytogenetic location: 1p36.11 Genomic coordinates (GRCh38) : 1:24,643,302-24,673,281 (from NCBI)
Pre-mRNA synthesis and processing involves factors widely distributed throughout the nucleus, with concentrations in 2 types of foci, speckles and coiled bodies. A subset of splicing factors in speckles is associated with the nuclear matrix. One of these, SRM160, forms a complex with SRM300 (606032) that functions as a pre-mRNA-specific splicing coordinator (Blencowe et al., 1998).
For background information on the SR family of serine/arginine-rich splicing factors, see 600812.
By biochemical purification, micropeptide sequence analysis, EST database searching, and screening a monocytoid cDNA library, Blencowe et al. (1998) isolated cDNAs encoding SRM160 (SR-related matrix protein of 160 kD) and several isoforms. The deduced 820-amino acid protein is rich in SR/RS repeats and proline residues. SRM160 also has multiple putative phosphorylation sites and SH3-binding motifs but no RNA-recognition domains. Northern blot analysis revealed expression of a ubiquitous, variably expressed 3.8-kb transcript and a more restricted 6.0-kb transcript. Immunoblot analysis showed that the recombinant protein was expressed as a 160-kD protein in nuclear extract and that it could be reduced to a 120-kD protein by phosphatase treatment. Confocal microscopy demonstrated expression of SRM160 in nuclear speckles. Immunoprecipitation analysis detected interaction of SRM160 with SRM300 (606032), a nuclear matrix antigen, and 2 other SR proteins, SRp40 (600914) and SRp75 (601940). EMSA analysis indicated that SRM160/SRM300 associates with pre-mRNA and promotes splicing through interactions with SR family proteins. The association between SRM160/SRM300 and pre-mRNA is dependent on RNU1 (180680) and is stabilized by RNU2 (180690).
The International Radiation Hybrid Mapping Consortium mapped the SRM160 gene to chromosome 1 (RH40469).
Blencowe, B. J., Issner, R., Nickerson, J. A., Sharp, P. A. A coactivator of pre-mRNA splicing. Genes Dev. 12: 996-1009, 1998. [PubMed: 9531537] [Full Text: https://doi.org/10.1101/gad.12.7.996]