Alternative titles; symbols
HGNC Approved Gene Symbol: SNAPC5
Cytogenetic location: 15q22.31 Genomic coordinates (GRCh38) : 15:66,489,748-66,497,762 (from NCBI)
SNAPC5 encodes a subunit of the snRNA-activating protein complex (SNAPc), which is required for transcription of both RNA polymerase II and III snRNA genes (see SNAPC1, 600591) (Henry et al., 1998).
By microsequence analysis of a 19-kD polypeptide present in SNAPC, followed by EST database searching and RT-PCR analysis, Henry et al. (1998) obtained a cDNA encoding SNAPC5, which they termed SNAP19. Sequence analysis predicted that the 98-amino acid protein contains a leucine zipper motif at its N terminus and a 10-residue glutamate stretch at its C terminus.
Using supershift and immunoblot analyses, Henry et al. (1998) confirmed the existence of SNAPC5 in the complex with the proximal sequence element (PSE) of snRNA promoters and that it is required for snRNA gene transcription by both pol II and pol III. Immunoprecipitation analysis of cotranslated SNAP genes suggested that SNAPC4 (602777) forms a backbone on which SNAPC2 (605076) and SNAPC5 assemble. The latter is essential for the subsequent assembly of SNAPC1 with SNAPC4, which is followed by the assembly of SNAPC3 (602348) with SNAPC1. Henry et al. (1998) reconstituted SNAPC from the 5 recombinant subunits and showed that this core complex binds to the PSE and directs both RNA pol II and pol III snRNA transcription.
Gross (2014) mapped the SNAPC5 gene to chromosome 15q22.31 based on an alignment of the SNAPC5 sequence (GenBank AF093593) with the genomic sequence (GRCh37).
Gross, M. B. Personal Communication. Baltimore, Md. 4/16/2014.
Henry, R. W., Mittal, V., Ma, B., Kobayashi, R., Hernandez, N. SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III. Genes Dev. 12: 2664-2672, 1998. [PubMed: 9732265] [Full Text: https://doi.org/10.1101/gad.12.17.2664]