Alternative titles; symbols
HGNC Approved Gene Symbol: CPSF2
Cytogenetic location: 14q32.12 Genomic coordinates (GRCh38) : 14:92,121,969-92,172,145 (from NCBI)
Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis (summary by Murthy and Manley, 1995). The CPSF2 gene encodes the 100-kD subunit of CPSF.
Jenny et al. (1994) cloned a cDNA encoding bovine Cpsf2. Northern blot analysis using bovine Cpsf2 coding sequences revealed expression of 5.6-, 4.6-, 3.8-, and 3.0-kb transcripts in HeLa cells. Immunoblot analysis showed expression of a 100-kD nuclear protein in bovine and human cells. Immunofluorescence microscopy demonstrated expression of both the 100-kD and the 160-kD (CPSF1; 606027) CPSF subunits in nucleoplasm but not in nucleoli.
Nagase et al. (2000) cloned a partial cDNA encoding 579 amino acids of human CPSF2, which they called KIAA1367, from a fetal brain cDNA library. The partial human protein is 99% identical to the 782-amino acid bovine protein. RT-PCR analysis revealed ubiquitous but variable expression of CPSF2, with highest levels in liver and testis.
Samiotaki et al. (2000) mapped the CPSF2 gene to 14q31.3 using radiation hybrid analysis.
Jenny, A., Hauri, H.-P., Keller, W. Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit. Molec. Cell. Biol. 14: 8183-8190, 1994. [PubMed: 7969155] [Full Text: https://doi.org/10.1128/mcb.14.12.8183-8190.1994]
Murthy, K. G. K., Manley, J. L. The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3-prime-end formation. Genes Dev. 9: 2672-2683, 1995. [PubMed: 7590244] [Full Text: https://doi.org/10.1101/gad.9.21.2672]
Nagase, T., Kikuno, R., Ishikawa, K., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 65-73, 2000. [PubMed: 10718198] [Full Text: https://doi.org/10.1093/dnares/7.1.65]
Samiotaki, M., Balatsos, N. A. A., Courtis, N., Tsiapalis, C. M. Assignment of the 100-kDa subunit of cleavage and polyadenylation specificity factor (CPSF2) to human chromosome 14q31.3 by radiation hybrid mapping. Cytogenet. Cell Genet. 90: 328-329, 2000. [PubMed: 11124543] [Full Text: https://doi.org/10.1159/000056798]