Alternative titles; symbols
HGNC Approved Gene Symbol: CGREF1
Cytogenetic location: 2p23.3 Genomic coordinates (GRCh38) : 2:27,099,353-27,119,128 (from NCBI)
Using differential RT-PCR analysis of rat embryo fibroblast cells containing a temperature-sensitive p53 (191170) allele, Madden et al. (1996) isolated several transcripts upregulated specifically in cells harboring functional p53 protein, including 2 novel transcripts that the authors termed cell growth regulatory genes 11 (CGR11) and 19 (CGR19; 606138). A full-length CGR11 cDNA cloned from a human fetal brain cDNA library encodes a deduced 301-amino acid protein with a highly conserved putative Ca(2+)-binding EF-hand motif. The human and rat CGR11 proteins share 65% overall sequence identity and nearly 100% in the EF-hand motif. The human protein has 4 clustered 17-amino acid repeats within the C terminus, suggesting a structure resembling 4 alpha-helical domains interrupted by distinct turns. Northern blot analysis of rat tissues demonstrated expression of CGR11 predominantly in whole brain and kidney, with limited expression in heart, lung, liver, and skeletal muscle and no expression in spleen and testis. Transfection assay experiments demonstrated that CGR11 is able to inhibit growth in several cell lines. Deletion mutant experiments suggested that the EF-hand domain is essential for the growth inhibitory activity.
Gross (2014) mapped the CGREF1 gene to chromosome 2p23.3 based on an alignment of the CGREF1 sequence (GenBank BC034764) with the genomic sequence (GRCh37).
Gross, M. B. Personal Communication. Baltimore, Md. 5/21/2014.
Madden, S. L., Galella, E. A., Riley, D., Bertelsen, A. H., Beaudry, G. A. Induction of cell growth regulatory genes by p53. Cancer Res. 56: 5384-5390, 1996. [PubMed: 8968090]