Alternative titles; symbols
HGNC Approved Gene Symbol: TGS1
Cytogenetic location: 8q12.1 Genomic coordinates (GRCh38) : 8:55,773,446-55,826,445 (from NCBI)
Nuclear receptor coactivators participate in the transcriptional activation of specific genes by nuclear receptors. Zhu et al. (2001) isolated a nuclear receptor coactivator-interacting protein, designated PIMT, from a human liver cDNA library by using the coactivator peroxisome proliferator-activated receptor-interacting protein (PRIP; 605299) as bait in a yeast 2-hybrid screen. The PIMT cDNA encodes an 852-amino acid protein containing a 9-amino acid methyltransferase motif I (VVDAFCGVG) and an invariant segment (GXXGXXI) found in K-homology motifs of many RNA-binding proteins. Northern blot analysis demonstrated ubiquitous expression of a 3.2-kb PIMT transcript, with highest expression in heart, skeletal muscle, kidney, liver, and placenta. Immunofluorescence studies showed that the PIMT and PRIP proteins are colocalized in the nucleus. PIMT binds 5-adenosyl-L-methionine, the methyl donor for the methyltransfer reaction, and it also binds RNA, suggesting that it is an RNA methyltransferase. Overexpression of PIMT enhances the transcriptional activity of PPARG (601487) and RXR (see 180245), and this enhancement is further stimulated by overexpression of PRIP, suggesting that PIMT is a component of nuclear receptor signal transduction that acts through PRIP.
Zhu et al. (2001) determined that the PIMT gene contains more than 13 exons and spans more than 40 kb.
By sequence analysis, Zhu et al. (2001) mapped the PIMT gene to chromosome 8q11.
Zhu, Y., Qi, C., Cao, W.-Q., Yeldandi, A. V., Rao, M. S., Reddy, J. K. Cloning and characterization of PIMT, a protein with a methyltransferase domain, which interacts with and enhances nuclear receptor coactivator PRIP function. Proc. Nat. Acad. Sci. 98: 10380-10385, 2001. [PubMed: 11517327] [Full Text: https://doi.org/10.1073/pnas.181347498]