Alternative titles; symbols
HGNC Approved Gene Symbol: MYCBP
Cytogenetic location: 1p34.3 Genomic coordinates (GRCh38) : 1:38,862,490-38,873,378 (from NCBI)
The MYCBP gene encodes a protein that binds to the N-terminal region of MYC (190080) and stimulates the activation of E box-dependent transcription by MYC.
The MYC protooncogene appears to play key roles in cell proliferation, differentiation, transformation, and apoptosis. A variety of functions of the MYC gene product are attributable to protein-protein interactions with various cellular factors. The N-terminal region is involved in all the biologic functions of MYC, and different proteins are therefore thought to interact with the N-terminal region of MYC to display different functions. Taira et al. (1998) cloned 2 cDNAs that encode a novel MYC-binding protein of 11 kD, which they designated AMY1 for 'associate of MYC.' A long form of 1622 bp (AMY1L) and a short form of 552 bp (AMY1S) were derived from alternative usage of polyadenylation signals. Northern blot analysis detected 2 mRNAs of 1.8 and 0.85 kb, corresponding to AMY1L and AMY1S, resectively. Both transcripts were highly expressed in heart, placenta, and pancreas, while AMY1L was dominant in skeletal muscle and kidney, and was also present at low levels in lung. Taira et al. (1998) found that AMY1 is bound via its C-terminal region to the N-terminal region of MYC (amino acids 58-148) corresponding to the transactivation domain. The AMY1 protein stimulates the activation of E box-dependent transcription by MYC. AMY1, which localizes mostly in the cytoplasm, translocates into the nucleus in the S phase of the cell cycle upon an increase of MYC expression, and may thus control the transcriptional activity of MYC.
To assess the molecular function of MYC-binding protein, Furusawa et al. (2001) performed a 2-hybrid screening of cDNAs encoding AMY1-binding proteins, with AMY1 as a bait using a human HeLa cDNA library. They found a clone encoding AKAP149 (AKAP1; 602449). AMY1 was found to bind in vitro and in vivo to the regulatory subunit II-binding region of AKAP1 and S-AKAP84, a splicing variant of AKAP149 expressed in the testis. AMY1 was expressed postmeiotically in the testis, as was also S-AKAP84. AMY1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively. These results suggested that AMY1 plays a role in spermatogenesis.
By FISH, Taira et al. (1998) mapped the MYCBP gene to 1p33-p32.2.
Furusawa, M., Ohnishi, T., Taira, T., Iguchi-Ariga, S. M. M., Ariga, H. AMY-1, a c-Myc-binding protein, is localized in the mitochondria of sperm by association with S-AKAP84, an anchor protein of cAMP-dependent protein kinase. J. Biol. Chem. 276: 36647-36651, 2001. [PubMed: 11483602] [Full Text: https://doi.org/10.1074/jbc.M103885200]
Taira, T., Maeda, J., Onishi, T., Kitaura, H., Yoshida, S., Kato, H., Ikeda, M., Tamai, K., Iguchi-Ariga, S. M. M., Ariga, H. AMY-1, a novel C-MYC binding protein that stimulates transcription activity of C-MYC. Genes Cells 3: 549-565, 1998. [PubMed: 9797456] [Full Text: https://doi.org/10.1046/j.1365-2443.1998.00206.x]