Alternative titles; symbols
HGNC Approved Gene Symbol: RAI14
Cytogenetic location: 5p13.2 Genomic coordinates (GRCh38) : 5:34,656,328-34,832,612 (from NCBI)
Nagase et al. (2000) isolated a cDNA encoding RAI14, which they called KIAA1334, from a size-fractionated fetal brain cDNA library.
Kutty et al. (2001) characterized the RAI14 gene, which they called NORPEG, from human retinal pigment epithelial cells (ARPE-19), in which its expression is induced by all-trans-retinoic acid. The predicted human and mouse proteins contain 980 and 979 amino acids, respectively, and share 84% sequence identity. Human RAI14 has a predicted molecular mass of 110 kD, a pI of 5.83, 6 potential N-glycosylation sites, several ankyrin repeats, and several coiled-coil helical domains. Northern blot analysis of human tissues detected expression of 2 transcripts of approximately 5 and 3 kb. Like the ARPE-19 cells, placenta showed an intense signal at 5 kb and a weak signal at 3 kb; the reverse was observed in testis, with an intense signal at 3 kb and a weak signal at 5 kb. RAI14 was also highly expressed in several human cancer cell lines. Expression studies showed that RAI14 localizes to the cytoplasm. Confocal microscopic analysis in ARPE-19 cells showed threadlike projections in the cytoplasm reminiscent of the cytoskeleton. The expression of Rai14 was detected in mouse embryo at embryonic day 9.5 by in situ hybridization, and the expression appeared to be developmentally regulated. In adult mouse, the highest level of expression was detected in the seminiferous tubules of testis.
Kutty et al. (2001) determined that the RAI14 gene contains 18 exons and spans more than 120 kb.
By somatic cell hybrid analysis and FISH, Kutty et al. (2001) mapped the RAI14 gene to chromosome 5p13.3-p13.2.
Kutty, R. K., Kutty, G., Samuel, W., Duncan, T., Bridges, C. C., El-Sherbeeny, A., Nagineni, C. N., Smith, S. B., Wiggert, B. Molecular characterization and developmental expression of NORPEG, a novel gene induced by retinoic acid. J. Biol. Chem. 276: 2831-2840, 2001. [PubMed: 11042181] [Full Text: https://doi.org/10.1074/jbc.M007421200]
Nagase, T., Kikuno, R., Ishikawa, K., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 65-73, 2000. [PubMed: 10718198] [Full Text: https://doi.org/10.1093/dnares/7.1.65]