Alternative titles; symbols
HGNC Approved Gene Symbol: NSFL1C
Cytogenetic location: 20p13 Genomic coordinates (GRCh38) : 20:1,442,166-1,466,849 (from NCBI)
The ATPases NSF (601633) and p97 (VCP; 601023) are involved in the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of membrane compartments. By biochemical purification of a 47-kD protein complexed with p97 in rat liver cytosol, followed by micropeptide sequence analysis and PCR with degenerate primers, Kondo et al. (1997) isolated a cDNA encoding rat p47. Rat p47 binds specifically to p97. Northern blot analysis revealed ubiquitous expression of a 1.5-kb p47 transcript in rat tissues. Kondo et al. (1997) also identified a human EST with approximately 89% sequence identity to rat p47. They concluded that the p97/p47 complex is probably the active component in the fusion reaction that leads to cisternal regrowth.
Zhang et al. (2000) screened CD34 (142230)-positive hemopoietic stem/progenitor cells for novel cDNAs and obtained a human homolog of p47. The deduced human p47 protein has 370 amino acids. Northern blot and macroarray analyses revealed abundant and ubiquitous expression of p47.
Zhang et al. (2000) determined that human p47 is encoded by a single exon.
Zhang et al. (2000) mapped the p47 gene to the distal end of chromosome 20p by STS analysis.
Kondo, H., Rabouille, C., Newman, R., Levine, T. P., Pappin, D., Freemont, P., Warren, G. p47 is a cofactor for p97-mediated membrane fusion. Nature 388: 75-78, 1997. [PubMed: 9214505] [Full Text: https://doi.org/10.1038/40411]
Zhang, Q.-H., Ye, M., Wu, X.-Y., Ren, S.-X., Zhao, M., Zhao, C.-J., Fu, G., Shen, Y., Fan, H.-Y., Lu, G., Zhong, M., Xu, X.-R., and 9 others. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res. 10: 1546-1560, 2000. [PubMed: 11042152] [Full Text: https://doi.org/10.1101/gr.140200]