Alternative titles; symbols
HGNC Approved Gene Symbol: MYDGF
Cytogenetic location: 19p13.3 Genomic coordinates (GRCh38) : 19:4,657,545-4,670,342 (from NCBI)
By profiling proteins secreted from preadipocytes (mouse 3T3-L1 cells) by means of 2-dimensional electrophoresis mass spectrometry, Wang et al. (2004) identified c19orf10 (SF10; IL25), which was upregulated during differentiation of adipocytes, leading to the suggestion that it is involved in adipogenesis.
During a proteomic analysis of fibroblast-like synoviocytes (FLSs), Weiler et al. (2007) identified C19ORF10, which was produced in significant amounts by these cells. The deduced 173-amino acid protein contains 2 O-glycosylation sites and several potential phosphorylation sites. There was evidence of 2 additional splice variants. The human and mouse C19ORF10 proteins share 91% sequence identity. Immunohistochemistry analyses of recombinant C19ORF10 in cultured FLSs and in synovial tissue sections from patients with rheumatoid arthritis (RA) or osteoarthritis (OA) revealed intracellular staining consistent with localization in the endoplasmic reticulum/Golgi apparatus. Sections of RA and OA synovia expressed similar patterns of C19ORF10 distribution with perivascular and synovial lining staining. Double-staining in situ analysis suggested that fibroblast-like synovial cells produced C19ORF10, whereas macrophages, B cells, or T cells produced little or none of this protein.
By surveying available cDNA clones, Weiler et al. (2007) found evidence of 3 splice variants of C19ORF10. The most common variant, C19ORF10b, has 6 exons and was supported by sequence data from 468 clones. The other 2 predicted variants, a and c, were supported by 1 clone each.
By database analysis, Weiler et al. (2007) mapped the C19ORF10 gene to chromosome 19p13.3.
Tulin et al. (2001) reported the cloning of full-length cDNAs encoding mouse and human SF20, which they also referred to as interleukin-25 (IL25). However, they retracted their paper in 2003.
Tulin, E. E., Onoda, N., Nakata, Y., Maeda, M., Hasegawa, M., Nomura, H., Kitamura, T. SF20/IL-25, a novel bone marrow stroma-derived growth factor that binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation. J. Immun. 167: 6338-6347, 2001. Note: Retraction: J. Immun. 170: 1593 only, 2003. [PubMed: 11714798] [Full Text: https://doi.org/10.4049/jimmunol.167.11.6338]
Wang, P., Mariman, E., Keijer, J., Bouwman, F., Noben, J.-P., Robben, J., Renes, J. Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines. Cell. Molec. Life Sci. 61: 2405-2417, 2004. [PubMed: 15378209] [Full Text: https://doi.org/10.1007/s00018-004-4256-z]
Weiler, T., Du, Q., Krokhin, O., Ens, W., Standing, K., El-Gabalawy, H., Wilkins, J. A. The identification and characterization of a novel protein, c19orf10, in the synovium. Arthritis Res. Ther. 9: R30, 2007. Note: Electronic Article. [PubMed: 17362502] [Full Text: https://doi.org/10.1186/ar2145]