Alternative titles; symbols
HGNC Approved Gene Symbol: CITED4
Cytogenetic location: 1p34.2 Genomic coordinates (GRCh38) : 1:40,861,054-40,862,363 (from NCBI)
CITED4 belongs to a family of transcriptional coactivators that bind several proteins, including CREB-binding protein (600140) and p300 (602700).
By database searching with sequences homologous to the p300 binding domain of CITED2 (602937), Braganca et al. (2002) identified a novel CITED EST which they used to obtain a genomic CITED4 clone. The deduced 184-amino acid protein contains the conserved 32-amino acid C-terminal motif of other CITED peptides. Northern blot analysis detected near ubiquitous expression of a 1.8-kb transcript, with highest levels in heart, liver, skeletal muscle, and pancreas. High expression was also found in several breast cancer cell lines and in a bladder cell line with endothelial characteristics. Endogenous CITED4, visualized by Western blot analysis, migrated at 24.5 kD. Confocal immunolocalization studies showed that CITED4 localizes in the nucleus in most cell lines, and in both the nucleus and cytoplasm in others.
By coimmunoprecipitation, in vitro translation, and mammalian 2-hybrid protein interaction experiments, Braganca et al. (2002) confirmed that CITED4 interacts with CBP/p300 through residues 138-184 of the C terminus. They also determined that CITED4 associates functionally with transcription factor AP2 (TFAP2; see 107580).
Braganca et al. (2002) determined that the CITED4 gene contains a single exon.
By FISH, Braganca et al. (2002) mapped the CITED4 gene to chromosome 1p35-p34.
Braganca, J., Swingler, T., Marques, F. I., Jones, T., Eloranta, J. J., Hurst, H. C., Shioda, T., Bhattacharya, S. Human CREB-binding protein/p300-interacting transactivator with ED-rich tail (CITED) 4, a new member of the CITED family, functions as a co-activator for transcription factor AP-2. J. Biol. Chem. 277: 8559-8565, 2002. [PubMed: 11744733] [Full Text: https://doi.org/10.1074/jbc.M110850200]