Alternative titles; symbols
HGNC Approved Gene Symbol: PLXDC1
Cytogenetic location: 17q12 Genomic coordinates (GRCh38) : 17:39,063,313-39,152,967 (from NCBI)
Using serial analysis of gene expression (SAGE), St. Croix et al. (2000) identified partial cDNAs corresponding to several tumor endothelial markers (TEMs) that displayed elevated expression during tumor angiogenesis. Among the genes they identified was PLXDC1, which they called TEM7. Using database searches and 5-prime RACE, Carson-Walter et al. (2001) derived sequences covering the entire TEM7 coding region, which encodes a 500-amino acid type I transmembrane protein containing a plexin-like domain. An alternate transcript of the TEM7 gene had been designated TEM3 by St. Croix et al. (2000). TEM3 and TEM7 differ in the use of alternative polyadenylation sites but result in the same predicted protein. The mouse ortholog of TEM7 shares 81% amino acid identity with the human protein.
In situ hybridization analysis of human colorectal cancer by Carson-Walter et al. (2001) demonstrated that TEM7 was expressed clearly in the endothelial cells of the tumor stroma but not in the endothelial cells of normal colonic tissue. Using in situ hybridization to assay expression in various normal adult mouse tissues, they observed that Tem7 was largely undetectable in mouse tissues or tumors, but was abundantly expressed in mouse brain. Carson-Walter et al. (2001) localized Tem7 expression to Purkinje cells of the cerebellum and some neuronal cells.
Stumpf (2022) mapped the PLXDC1 gene to chromosome 17q12 based on an alignment of the PLXDC1 sequence (GenBank AK312999) with the genomic sequence (GRCh38).
Carson-Walter, E. B., Watkins, D. N., Nanda, A., Vogelstein, B., Kinzler, K. W., St. Croix, B. Cell surface tumor endothelial markers are conserved in mice and humans. Cancer Res. 61: 6649-6655, 2001. [PubMed: 11559528]
St. Croix, B., Rago, C., Velculescu, V., Traverso, G., Romans, K. E., Montgomery, E., Lal, A., Riggins, G. J., Lengauer, C., Vogelstein, B., Kinzler, K. W. Genes expressed in human tumor endothelium. Science 289: 1197-1202, 2000. [PubMed: 10947988] [Full Text: https://doi.org/10.1126/science.289.5482.1197]
Stumpf, A. M. Personal Communication. Baltimore, Md. 01/18/2022.