Alternative titles; symbols
HGNC Approved Gene Symbol: RBM5
Cytogenetic location: 3p21.31 Genomic coordinates (GRCh38) : 3:50,088,919-50,119,021 (from NCBI)
Timmer et al. (1999) identified a YAC clone corresponding to chromosome 3p21.3 that is deleted in the small cell lung cancer cell line GLC20. They isolated and cloned RBM5 by direct hybridization of this YAC clone to a pre-B-cell cDNA library. The deduced 815-amino acid protein contains 2 RNA-binding motifs, a C2C2-type zinc finger motif, a C2H2-type zinc finger motif, and a bipartite nuclear signal. The same functional motifs are found in RBM6 (606886), as has also been reported by Gure et al. (1998), and the 2 proteins share 30% amino acid identity. Northern blot analysis of multiple tissues detected expression of 7.4- and 3.5-kb transcripts in all tissues investigated. Although the RBM5 gene is located in a region considered critical for the development of lung cancer, Northern and Southern analyses of a number of lung cancer cell lines revealed no patterns of aberrant RBM5 expression.
By physical cloning methodologies and bioinformatic computational analyses, Lerman and Minna (2000) identified a number of genes, including RBM5, which they called G15, in a region of chromosome 3p21.3 that is associated with a putative lung cancer tumor suppressor gene. The mouse ortholog is 97% identical on the protein level. Northern blot analysis detected wide expression in normal and lung cancer tissues and cell lines.
Drabkin et al. (1999) found that recombinant proteins containing the RNA recognition motifs of RBM5 or RBM6 specifically bind poly(G) RNA homopolymers in vitro.
Lerman and Minna (2000) identified no mutations in the RBM5 gene in lung cancer cell lines. They concluded that RBM5 is an unlikely candidate as a tumor suppressor gene.
Oh et al. (2002) found that the RBM5 gene, which they called H37, showed reduced expression in 9 of 11 primary nonsmall cell lung cancers when compared with adjacent normal bronchial cells. Introduction of H37 cDNA into human breast cancer cells with deletion of 3p22-p21 reduced both anchorage-dependent and -independent cell growth in vitro. They concluded that H37 may have a role as a tumor suppressor gene in human lung cancer.
Timmer et al. (1999) determined that the RBM5 gene contains 25 exons. By genomic sequence analysis, Lerman and Minna (2000) detected only 18 exons in the 18.5-kb RBM5 gene.
Timmer et al. (1999) identified the RBM5 gene within a YAC clone corresponding to chromosome 3p21.3.
Drabkin, H. A., West, J. D., Hotfilder, M., Heng, Y. M., Erickson, P., Calvo, R., Dalmau, J., Gemmill, R. M., Sablitzky, F. DEF-3(g16/NY-LU-12), an RNA binding protein from the 3p21.3 homozygous deletion region in SCLC. Oncogene 18: 2589-2597, 1999. [PubMed: 10353602] [Full Text: https://doi.org/10.1038/sj.onc.1202601]
Gure, A. O., Altorki, N. K., Stockert, E., Scanlan, M. J., Old, L. J., Chen, Y.-T. Human lung cancer antigens recognized by autologous antibodies: definition of a novel cDNA derived from the tumor suppressor gene locus on chromosome 3p21.3. Cancer Res. 58: 1034-1041, 1998. [PubMed: 9500467]
Lerman, M. I., Minna, J. D. The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes. Cancer Res. 60: 6116-6133, 2000. [PubMed: 11085536]
Oh, J. J., West, A. R., Fishbein, M. C., Slamon, D. J. A candidate tumor suppressor gene, H37, from the human lung cancer tumor suppressor locus 3p21.3. Cancer Res. 62: 3207-3213, 2002. [PubMed: 12036935]
Timmer, T., Terpstra, P., van den Berg, A., Veldhuis, P. M. J. F., Elst, A. T., Voutsinas, G., Hulsbeek, M. M. F., Draaijers, T. G., Looman, M. W. G., Kok, K., Naylor, S. L., Buys, C. H. C. M. A comparison of genomic structures and expression patterns of two closely related flanking genes in a critical lung cancer region at 3p21.3. Europ. J. Hum. Genet. 7: 478-486, 1999. [PubMed: 10352938] [Full Text: https://doi.org/10.1038/sj.ejhg.5200334]