HGNC Approved Gene Symbol: VAMP4
Cytogenetic location: 1q24.3 Genomic coordinates (GRCh38) : 1:171,700,160-171,742,065 (from NCBI)
VAMP4 is a member of the vesicle-associated membrane protein family of trafficking proteins.
Advani et al. (1998) used an EST identified through a database search to obtain a corresponding human VAMP4 germinal center B-cell clone. The predicted 141-amino acid protein contains an amphipathic helix that may participate in coiled-coil interactions and a C-terminal hydrophobic domain predicted to serve as a membrane anchor. Northern blot analysis detected transcripts of 1.4, 2.4, and 5.6 kb that are broadly expressed and abundant in heart. Transfection into normal rat kidney cells resulted in a punctate juxtanuclear staining pattern that partially overlaps with syntaxin-6 (STX6; 603944), a marker for the trans-Golgi network. By Western blot analysis, Steegmaier et al. (1999) identified a single 18-kD protein expressed in all rat tissues examined except skeletal muscle, with highest expression in brain and testis. They also found expression in all mammalian cell lines tested. By immunoprecipitation of rat brain detergent extracts, Steegmaier et al. (1999) found that VAMP4 exists in a complex with syntaxin-6.
Steegmaier et al. (1999) suggested that VAMP4 plays a role in trans-Golgi network-to-endosome transport.
The International Radiation Hybrid Mapping Consortium mapped the VAMP4 gene to chromosome 1 (stSG54007). Scott (2002) mapped VAMP4 to 1q24-q25 based on sequence similarity between VAMP4 (GenBank AF044310) and a BAC (GenBank AL035296) mapped to this location.
Advani, R. J., Bae, H.-R., Bock, J. B., Chao, D. S., Doung, Y.-C., Prekeris, R., Yoo, J.-S., Scheller, R. H. Seven novel mammalian SNARE proteins localize to distinct membrane compartments. J. Biol. Chem. 273: 10317-10324, 1998. [PubMed: 9553086] [Full Text: https://doi.org/10.1074/jbc.273.17.10317]
Scott, A. F. Personal Communication. Baltimore, Md. 5/6/2002.
Steegmaier, M., Klumperman, J., Foletti, D. L., Yoo, J.-S., Scheller, R. H. Vesicle-associated membrane protein 4 is implicated in trans-Golgi network vesicle trafficking. Molec. Biol. Cell 10: 1957-1972, 1999. [PubMed: 10359608] [Full Text: https://doi.org/10.1091/mbc.10.6.1957]