HGNC Approved Gene Symbol: ZNF253
Cytogenetic location: 19p13.11 Genomic coordinates (GRCh38) : 19:19,865,830-19,894,674 (from NCBI)
Using PCR primers based on conserved sequences of Kruppel-like genes to screen a bone marrow cDNA library, followed by database searching and 3-prime and 5-prime RACE, Han et al. (1999) isolated cDNAs encoding several zinc finger proteins, including ZNF253. The deduced 275-amino acid ZNF253 protein contains 3 tandemly repeated C-terminal zinc finger motifs with interspersed conserved knuckle sequences, as well as an N-terminal Kruppel-associated box (KRAB). RT-PCR analysis detected selective expression restricted to monocytic and erythroid cell lines within the hematopoietic system.
By functional analysis, Han et al. (1999) showed that the KRAB box of ZNF253 has a transcriptional repressor effect in both yeast and mammalian cells.
Using FISH, Han et al. (1999) mapped the ZNF253 gene to chromosome 19p13, where the ZNF254 (604768) and ZNF257 (606957) genes also map.
Han, Z.-G., Zhang, Q.-H., Ye, M., Kan, L.-X., Gu, B.-W., He, K.-L., Shi, S.-L., Zhou, J., Fu, G., Mao, M., Chen, S.-J., Yu, L., Chen, Z. Molecular cloning of six novel Kruppel-like zinc finger genes from hematopoietic cells and identification of a novel transregulatory domain KRNB. J. Biol. Chem. 274: 35741-35748, 1999. [PubMed: 10585455] [Full Text: https://doi.org/10.1074/jbc.274.50.35741]