Alternative titles; symbols
HGNC Approved Gene Symbol: VAMP5
Cytogenetic location: 2p11.2 Genomic coordinates (GRCh38) : 2:85,584,431-85,593,406 (from NCBI)
By database searching with the mouse synaptobrevin/Vamp2 (185881) sequence as query, Zeng et al. (1998) identified a mouse EST clone for Vamp5. The deduced 102-amino acid protein is predicted to contain a cytoplasmic coiled-coil structure at the N terminus, a hydrophobic domain, and a short hydrophilic C-terminal sequence. Vamp5 shares 33% sequence identity with cellubrevin (VAMP3; 603657) and synaptobrevin/Vamp2. Northern blot analysis detected a major 700-bp transcript in skeletal muscle and heart, with much lower expression in spleen, lung, liver, kidney, and testis, and no expression in brain. An approximately 1.5-kb transcript was detected in testis. By Northern blot analysis of mouse muscle cells in culture, Zeng et al. (1998) found that Vamp5 expression was 8- to 10-fold higher in multinucleated myotubes than in undifferentiated myoblasts. Western blot analysis revealed protein levels 6-fold higher in myotubes than in myoblasts. Immunofluorescence and electron microscopy indicated that Vamp5 localizes to intracellular perinuclear and peripheral vesicular structures of myotubes as well as to the plasma membrane. Vamp5 staining did not colocalize with a Golgi marker, GS28 (GOSR1; 604026), upon treatment of the cells with Golgi-disrupting agents.
The International Radiation Hybrid Mapping Consortium mapped the VAMP5 gene to chromosome 2 (SGC32754).
Zeng, Q., Subramaniam, V. N., Wong, S. H., Tang, B. L., Parton, R. G., Rea, S., James, D. E., Hong, W. A novel synaptobrevin/VAMP homologous protein (VAMP5) is increased during in vitro myogenesis and present in the plasma membrane. Molec. Biol. Cell 9: 2423-2437, 1998. [PubMed: 9725904] [Full Text: https://doi.org/10.1091/mbc.9.9.2423]