Alternative titles; symbols
HGNC Approved Gene Symbol: CCNDBP1
Cytogenetic location: 15q15.2 Genomic coordinates (GRCh38) : 15:43,185,403-43,197,177 (from NCBI)
In a yeast 2-hybrid screen with the C-terminal glu/pro-rich domain and the SH3 domain (QC domain) of GRAP2 (604518) as bait, Xia et al. (2000) cloned CCNDBP1, which they called GCIP, from a pretransformed human bone marrow cDNA library. CCNDBP1 encodes a deduced 360-amino acid protein with a calculated molecular mass of 40 kD. It is predicted to contain a helix-loop-helix region without the basic DNA-binding domain, a central acidic domain, and a leucine zipper motif. Northern blot analysis detected a 1.3-kb transcript in all tissues examined, with highest expression in heart, muscle, peripheral leukocytes, kidney, and brain. RT-PCR revealed expression in all immune tissues, with relatively low expression in bone marrow.
Xia et al. (2000) verified interaction between GCIP and the QC domain of GRAP2 in yeast 2 hybrid assays, in in vitro binding assays, and by Western blot analysis of immunoprecipitates of cotransfected COS-7 cells. GST pull-down assays of cell lysates from several cell lines and coimmunoprecipitation of cyclin D with GCIP from GCIP-transfected cells showed that GCIP also associates with cyclin D (CCND1; 168461). Overexpression of GCIP in COS-7 cells reduced the phosphorylation of the retinoblastoma protein (RB1; 614041) by immunopurified cyclin D/CDK4 (123829) complexes. GCIP expression also reduced the transcriptional activity of a cotransfected E2F1 (189971) reporter plasmid.
The International Radiation Hybrid Mapping Consortium mapped the CCNDBP1 gene to chromosome 15 (WI16359).
Xia, C., Bao, Z., Tabassam, F., Ma, W., Qiu, M., Hua, S., Liu, M. GCIP, a novel human Grap2 and cyclin D interacting protein, regulates E2F-mediated transcriptional activity. J. Biol. Chem. 275: 20942-20948, 2000. [PubMed: 10801854] [Full Text: https://doi.org/10.1074/jbc.M002598200]