Alternative titles; symbols
HGNC Approved Gene Symbol: TNKS1BP1
Cytogenetic location: 11q12.1 Genomic coordinates (GRCh38) : 11:57,299,642-57,324,952 (from NCBI)
Using tankyrase-1 (TNKS; 603303) as bait in a yeast 2-hybrid screen, Seimiya and Smith (2002) cloned TAB182 from a fetal liver cDNA library. The deduced 1,729-amino acid protein has a calculated molecular mass of 182 kD. It is predicted to contain 2 basic regions at its N and C termini and a large internal acidic region. The C terminus also contains 2 potential nuclear localization signals. Northern blot analysis detected transcripts of 7, 4.4, and 2.3 kb in multiple tissues, with highest expression in testis, ovary, and lung, and minimal expression in brain and peripheral blood leukocytes. By immunolocalization of endogenous TAB182 in 2 human cell lines, Seimiya and Smith (2002) found TAB182 in the cytoplasm, where it colocalized with cortical actin, and in the nucleus. The nuclear staining was similar to that found for heterochromatin proteins. In a synchronized population of HeLa cells, TAB182 associated with chromosomes throughout mitosis.
Using several TAB182 deletion mutants, Seimiya and Smith (2002) localized the tankyrase-1-binding domain to the carboxy end of the acidic domain of TAB182. This region was found to bind the ankyrin repeat domain of tankyrase-1 and tankyrase-2 (607128), but not to that of ankyrin-G (ANK3; 600465). They also found that TAB182 is a substrate for tankyrase-1 poly(ADP-ribosyl)ation.
Seimiya and Smith (2002) mapped the TNKS1BP1 gene to chromosome 11q12 based on its sequence similarity with a genomic clone mapped to this location.
Seimiya, H., Smith, S. The telomeric poly(ADP-ribose) polymerase, tankyrase 1, contains multiple binding sites for telomeric repeat binding factor 1 (TRF1) and a novel acceptor, 182-kDa tankyrase-binding protein (TAB182). J. Biol. Chem. 277: 14116-11426, 2002. [PubMed: 11854288] [Full Text: https://doi.org/10.1074/jbc.M112266200]