Alternative titles; symbols
HGNC Approved Gene Symbol: TLN2
Cytogenetic location: 15q22.2 Genomic coordinates (GRCh38) : 15:62,390,550-62,844,631 (from NCBI)
By randomly sequencing clones from a brain cDNA library, Nagase et al. (1997) obtained a partial cDNA encoding TLN2, which they called KIAA0320. RT-PCR detected expression in heart, brain, kidney, thymus, testis, and small intestine.
Using genomic and cDNA/EST sequences, Monkley et al. (2001) assembled the complete coding sequence for TLN2. The deduced 2,532-amino acid protein contains a FERM domain. In TLN1 (186745), the FERM domain binds the cytodomains of beta-1 (135630) and beta-3 (173470) integrins, as well as to F-actin (see 102610) and other proteins. TLN2 shares 74% identity with TLN1, and the N- and C-terminal regions of TLN2 are highly conserved across species. Analysis of EST databases indicated that both TLN1 and TLN2 are expressed in brain, lung, heart, eye, mammary gland, colon, stomach, lymphocytes, germ cells, liver, spleen, bone, and many tumors. Northern blot analysis of adult mouse tissues revealed multiple Tln2 transcripts, with highest expression in heart and undetectable expression in spleen.
Di Paolo et al. (2002) and Ling et al. (2002) presented evidence that talin, through its FERM domain, interacts with the C-terminal tail of the 90-kD PIP5K1C (606102) isoform. Di Paolo et al. (2002) determined that, in rat brain, the predominant talin that interacts with PIP5K1C is Tln2. The authors showed that this interaction induces clustering of PIP5K1C and talin at focal adhesions and increases the local production of phosphatidylinositol-4,5-bisphosphate.
Monkley et al. (2001) determined that the TLN2 gene contains 55 exons and spans about 190 kb.
Chu et al. (2014) identified the gene encoding microRNA-190A (MIR190A; 615845) within an intron of the TLN2 gene. However, they found that MIR190A expression was independent of TLN2 expression.
By radiation hybrid analysis, Nagase et al. (1997) mapped the TLN2 gene to chromosome 15. By genomic sequence analysis, Monkley et al. (2001) mapped the TLN2 gene to chromosome 15q15-q21. Chu et al. (2014) mapped the TLN2 gene to chromosome 15q22.2 by genomic sequence analysis.
Chu, H.-W., Cheng, C.-W., Chou, W.-C., Hu, L.-Y., Wang, H.-W., Hsiung, C.-N., Hsu, H.-M., Wu, P.-E., Hou, M.-F., Shen, C.-Y., Yu, J.-C. A novel estrogen receptor-microRNA 190a-PAR-1-pathway regulates breast cancer progression, a finding initially suggested by genome-wide analysis of loci associated with lymph-node metastasis. Hum. Molec. Genet. 23: 355-367, 2014. [PubMed: 24009311] [Full Text: https://doi.org/10.1093/hmg/ddt426]
Di Paolo, G., Pellegrini, L., Letinic, K., Cestra, G., Zoncu, R., Voronov, S., Chang, S., Guo, J., Wenk, M. R., De Camill, P. Recruitment and regulation of phosphatidylinositol phosphate kinase type I-gamma by the FERM domain of talin. Nature 420: 85-89, 2002. [PubMed: 12422219] [Full Text: https://doi.org/10.1038/nature01147]
Ling, K., Doughman, R. L., Firestone, A. J., Bunce, M. W., Anderson, R. A. Type I-gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions. Nature 420: 89-93, 2002. [PubMed: 12422220] [Full Text: https://doi.org/10.1038/nature01082]
Monkley, S. J., Pritchard, C. A., Critchley, D. R. Analysis of the mammalian talin2 gene TLN2. Biochem. Biophys. Res. Commun. 286: 880-885, 2001. [PubMed: 11527381] [Full Text: https://doi.org/10.1006/bbrc.2001.5497]
Nagase, T., Ishikawa, K., Nakajima, D., Ohira, M., Seki, N., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 4: 141-150, 1997. [PubMed: 9205841] [Full Text: https://doi.org/10.1093/dnares/4.2.141]