Alternative titles; symbols
HGNC Approved Gene Symbol: SERBP1
Cytogenetic location: 1p31.3 Genomic coordinates (GRCh38) : 1:67,407,810-67,430,402 (from NCBI)
Heaton et al. (2001) purified Pairbp1 from rat hepatoma cells and determined that it binds to the cyclic nucleotide-responsive element of the rat Pai1 gene (173360). Based on homology with the N-terminal amino acid sequence of the rat protein, they identified a human PAIRBP1 cDNA. The deduced 387-amino acid human protein does not contain an RNA recognition motif or a K homology domain, but it has other motifs frequently found in RNA-binding proteins, notably an RGG box, an RG-rich region, and an arg-rich region. It also contains a putative protein kinase A (see 601639) phosphorylation site at ser74. PAIRBP1 shares several blocks of conserved sequence with proteins from Arabidopsis, Drosophila, chicken, mouse, and rat.
Using RNA electrophoretic mobility shift assay and ultraviolet cross-linking experiments, Heaton et al. (2001) determined that recombinant PAIRBP1 binds to the cyclic nucleotide-responsive element of rat Pai1 in a concentration-dependent manner. By mutation analysis, they determined that PAIRBP1 interacts primarily with the adenine-rich region of the response element.
Gross (2014) mapped the SERBP1 gene to chromosome 1p31.3 based on an alignment of the SERBP1 sequence (GenBank AF151813) with the genomic sequence (GRCh37).
Gross, M. B. Personal Communication. Baltimore, Md. 5/29/2014.
Heaton, J. H., Dlakic, W. M., Dlakic, M., Gelehrter, T. D. Identification and cDNA cloning of a novel RNA-binding protein that interacts with the cyclic nucleotide-responsive sequence in the type-1 plasminogen activator inhibitor mRNA. J. Biol. Chem. 276: 3341-3347, 2001. [PubMed: 11001948] [Full Text: https://doi.org/10.1074/jbc.M006538200]